Proteome approach in identification of novel tumour markers in patients with cancer

Pao Yue-kong Library Electronic Theses Database

Proteome approach in identification of novel tumour markers in patients with cancer

 

Author: Yong, Fung-mei
Title: Proteome approach in identification of novel tumour markers in patients with cancer
Degree: M.Sc.
Year: 2001
Subject: Tumor markers
Hong Kong Polytechnic University -- Dissertations
Department: Multi-disciplinary Studies
Dept. of Applied Biology and Chemical Technology
Pages: ix, 83 leaves : ill. (some col.) ; 30 cm
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b1553351
URI: http://theses.lib.polyu.edu.hk/handle/200/1091
Abstract: Current common diagnostic methods for tumour are either invasive in nature or involving exposure to radiation. Compared with healthy individuals, tumour patients have elevated level of some proteins in serum due to uncontrolled proliferation. Presence of these proteins can be used as tumour markers. This work involves studies of the changes in two-dimensional polyacrylamde gel electrophoresis (2D-PAGE) patterns of the sera samples from patients with lung carcinoma, stomach carcinoma and colon carcinoma. The aim is to identify new tumour markers in order to facilitate early detection and diagnosis. Tumour marker is defined as the protein spot only present in the 2D-PAGE gels of sera from tumour patients but not from healthy individuals. It is found that there are five probable tumour markers (pI/Mr,=4.8/60kDa, 5.5/60kDa, 5.6/60kDa, 5.6/52kDa, 5.7/52kDa) for stomach adenocarcinoma. Nine probable markers are found (4.8/60kDa, 5.4/60kDa, 5.5/60kDa, 5.6/60kDa, 5.5/52kDa 5.6/52kDa 5.7/52kDa 6.4/45kDa, 6.5/45kDa) for stomach adenosquamous carcinoma; two others (5.7/60kDa, 5.9/52kDa) for colon adenocarcinoma. In addition, three markers (4.8/60kDa, 5.2/60kDa 5.7/52kDa) for squamous cell lung carcinoma and six (4.8/60kDa, 6.5/56kDa, 6.6/54kDa, 6.8/55kDa, 5.7/52kDa 6.0/28kDa) for large cell lung anaplastic carcinoma. Among these, it can be found that all the sera samples with carcinoma appear to have a same spot with a pI/Mr of 5.7/52kDa. Therefore the spot may be a general marker for occurrence of tumour. Moreover, other protein spots with pI 5.8-6.0, Mr 45kDa are commonly present in normal serum but absent in 7 out of 9 tumour images. Furthermore the spot at 4.8/60kDa is present in two cases in stomach carcinoma and two cases in lung carcinoma but not the others and yet another spot at 5.4/55kDa is absent in some cases randomly. Further investigation to identify these markers is necessary.

Files in this item

Files Size Format
b15533517.pdf 5.332Mb PDF
Copyright Undertaking
As a bona fide Library user, I declare that:
  1. I will abide by the rules and legal ordinances governing copyright regarding the use of the Database.
  2. I will use the Database for the purpose of my research or private study only and not for circulation or further reproduction or any other purpose.
  3. I agree to indemnify and hold the University harmless from and against any loss, damage, cost, liability or expenses arising from copyright infringement or unauthorized usage.
By downloading any item(s) listed above, you acknowledge that you have read and understood the copyright undertaking as stated above, and agree to be bound by all of its terms.

     

Quick Search

Browse

More Information