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dc.contributorDepartment of Health Technology and Informaticsen_US
dc.contributor.advisorZou, Xiang (HTI)en_US
dc.creatorLam, Ka Wai-
dc.identifier.urihttps://theses.lib.polyu.edu.hk/handle/200/12073-
dc.languageEnglishen_US
dc.publisherHong Kong Polytechnic Universityen_US
dc.rightsAll rights reserveden_US
dc.titleDamage-associated molecular pattern S100A4 : a potential adjuvant for mucosal immunizationen_US
dcterms.abstractIntroductionen_US
dcterms.abstractPathogenic microorganisms, including bacteria, viruses, fungi and parasites, can enter the human body through different routes. Mucosal membrane is often the first site of entry although different types of mucosa-associated lymphoid tissue (MALT) responsible for providing physical, chemical and immunological protection against foreign pathogens are present in the human body. Mucosal infections, such as influenza and COVID-19 pandemic, have been causing high morbidity and mortality in humans. Mucosal immunization is an effective strategy for prevention of mucosal infections. However, due to the immunotolerance effect produced by mucosal membranes, the immunogenicity of mucosal vaccine is unacceptably low. The use of adjuvant may be a possible solution to this inherent problem. Among different adjuvants, the damage-associated molecular pattern (DAMP) molecule S100A4 may be a potential candidate for eliciting a strong mucosal immune response.en_US
dcterms.abstractAimsen_US
dcterms.abstractThis study aims at investigating the effects of DAMP molecule S100A4 as an mucosal adjuvant for intranasal immunization.en_US
dcterms.abstractMethodsen_US
dcterms.abstractMice were intranasally immunized three times with model antigen ovalbumin (OVA; 10 μg) alone, or admixed to S100A4 (10 μg) or cholera toxin (CT; 1 μg) at a 10-day interval and blood and lung samples were collected 10 days after the last immunization for investigation. Two experiments were performed on the serum and lung samples. Enzyme-linked immunosorbent assay (ELISA) was performed to measure the serum OVA-specific total IgG and IgG subclass (IgG1 and IgG2c) antibody titers. Flow cytometry analysis was performed to measure the activation of OVA-specific cytotoxic T lymphocytes.en_US
dcterms.abstractResultsen_US
dcterms.abstractAnti-OVA total IgG, IgG1 and IgG2c levels in mice serum were significantly enhanced after immunization adjuvanted with the DAMP molecule S100A4. S100A4 as an adjuvant after intranasal immunization also augmented the antigen specific cytotoxic T lymphocytes activation in the lungs.en_US
dcterms.abstractConclusionen_US
dcterms.abstractThe results suggested that intranasal immunization adjuvanted with S100A4 can trigger greater humoral and cellular immune response. However, further studies are needed more convincing conclusion.en_US
dcterms.extentix, 64 pages : color illustrationsen_US
dcterms.isPartOfPolyU Electronic Thesesen_US
dcterms.issued2022en_US
dcterms.educationalLevelM.Sc.en_US
dcterms.educationalLevelAll Masteren_US
dcterms.LCSHMucous membrane -- Immunologyen_US
dcterms.LCSHImmunological adjuvantsen_US
dcterms.LCSHVaccinesen_US
dcterms.LCSHHong Kong Polytechnic University -- Dissertationsen_US
dcterms.accessRightsrestricted accessen_US

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Please use this identifier to cite or link to this item: https://theses.lib.polyu.edu.hk/handle/200/12073