Establishment and optimization of a method for the determination of mycophenolic acid in plasma of local renal allograft recipients

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Establishment and optimization of a method for the determination of mycophenolic acid in plasma of local renal allograft recipients

 

Author: Soo, Yin-chu
Title: Establishment and optimization of a method for the determination of mycophenolic acid in plasma of local renal allograft recipients
Degree: M.Sc.
Year: 1999
Subject: Kidneys -- Transplantation -- China -- Hong Kong -- Immunological aspects
Immunosuppressive agents -- China -- Hong Kong -- Pharmacokinetics
Hong Kong Polytechnic University -- Dissertations
Department: Multi-disciplinary Studies
Dept. of Nursing and Health Sciences
Pages: xi, 105 leaves : ill. ; 30 cm
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b1477327
URI: http://theses.lib.polyu.edu.hk/handle/200/1971
Abstract: Mycophenolate mofetil (MMF) is one of the latest member of modern immunosuppressants. With or without combination of other immunosuppressive agents, it may enable increased efficacy without any increased toxicity. Although mycophenolic acid (MPA) has been studied overseas, there is no pharmacokinetic data in local population. Plasma MPA concentration profiles for patients after renal transplantation will be monitored for three months and assayed by high-performance liquid chromatography (HPLC). The determination of its peak (Cmax) and trough (Cmin) levels and area under MPA plasma concentration-time curve (AUC) for local patients facilitates development of therapeutic drug monitoring protocol. The laboratory method developed had a short retention time, 5.97 minutes for internal standard and 6.98 minutes for MPA. Intra-assay coefficient of variation CV (n=10) was<3.74% and inter-assay CV (n=30) was <6.46% over the range of 1.0-20.0 mg/L. Linearity was up to 40.0 mg/L and detection limit 0.2 mg/L. Medications that are commonly used among renal patients such as dothiepin, imipramine, naproxen, prednisolone, midazolam, carbamazepine, paroxetine, verapamil, propanolol, frusemide. methyl salicylate, indomethacin and phenytoin showed no interference. It may be put into routine service in the future for the management of patients after renal transplantation. In this project, no significant difference could be detected statistically for MPA Cmin, Cmax, time to Cmax (Tmax) and AUC between different periods of post transplantation. However, MPA AUC was found to have a positive correlation with Cmin and Cmax but a negative relation with Tmax.

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