| Author: | Soo, Yin-chu |
| Title: | Establishment and optimization of a method for the determination of mycophenolic acid in plasma of local renal allograft recipients |
| Degree: | M.Sc. |
| Year: | 1999 |
| Subject: | Kidneys -- Transplantation -- China -- Hong Kong -- Immunological aspects Immunosuppressive agents -- China -- Hong Kong -- Pharmacokinetics Hong Kong Polytechnic University -- Dissertations |
| Department: | Multi-disciplinary Studies Department of Nursing and Health Sciences |
| Pages: | xi, 105 leaves : ill. ; 30 cm |
| Language: | English |
| Abstract: | Mycophenolate mofetil (MMF) is one of the latest member of modern immunosuppressants. With or without combination of other immunosuppressive agents, it may enable increased efficacy without any increased toxicity. Although mycophenolic acid (MPA) has been studied overseas, there is no pharmacokinetic data in local population. Plasma MPA concentration profiles for patients after renal transplantation will be monitored for three months and assayed by high-performance liquid chromatography (HPLC). The determination of its peak (Cmax) and trough (Cmin) levels and area under MPA plasma concentration-time curve (AUC) for local patients facilitates development of therapeutic drug monitoring protocol. The laboratory method developed had a short retention time, 5.97 minutes for internal standard and 6.98 minutes for MPA. Intra-assay coefficient of variation CV (n=10) was<3.74% and inter-assay CV (n=30) was <6.46% over the range of 1.0-20.0 mg/L. Linearity was up to 40.0 mg/L and detection limit 0.2 mg/L. Medications that are commonly used among renal patients such as dothiepin, imipramine, naproxen, prednisolone, midazolam, carbamazepine, paroxetine, verapamil, propanolol, frusemide. methyl salicylate, indomethacin and phenytoin showed no interference. It may be put into routine service in the future for the management of patients after renal transplantation. In this project, no significant difference could be detected statistically for MPA Cmin, Cmax, time to Cmax (Tmax) and AUC between different periods of post transplantation. However, MPA AUC was found to have a positive correlation with Cmin and Cmax but a negative relation with Tmax. |
| Rights: | All rights reserved |
| Access: | restricted access |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| b14773272.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 2.62 MB | Adobe PDF | View/Open |
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