The effects of Chinese herbal medicines on the crystallization of calcium oxalate in an in-vitro artificial crystalliser and by 96-well plate oxalate induced turbidity method

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The effects of Chinese herbal medicines on the crystallization of calcium oxalate in an in-vitro artificial crystalliser and by 96-well plate oxalate induced turbidity method

 

Author: Chan, Kin-ping
Title: The effects of Chinese herbal medicines on the crystallization of calcium oxalate in an in-vitro artificial crystalliser and by 96-well plate oxalate induced turbidity method
Degree: M.Sc.
Year: 2006
Subject: Hong Kong Polytechnic University -- Dissertations.
Kidneys -- Calculi -- Prevention.
Kidneys -- Calculi -- Treatment.
Medicine, Chinese.
Herbs -- Therapeutic use.
Department: Dept. of Health Technology and Informatics
Pages: ix, 92 leaves : ill. ; 30 cm.
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b2174225
URI: http://theses.lib.polyu.edu.hk/handle/200/2035
Abstract: The aim of this study was to examine the effects of Herba Lysimachiae (金錢草) and Folium Pyrrosiae (石葦) on the nucleation and growth of calcium oxalate crystals by continuous flow crystallizer system called Mixed Suspension and Mixed Product Removal (MSMPR) and by oxalate induced turbidity method. In this study, concentrated powder form of the herbs was used to make three different concentrations which are 1.0 g/L, 0.5 g/L and 0.1 g/L. The results by the MSMPR demonstrated that Herba Lysimachiae had a strong inhibitory effect on the growth rate of calcium oxalate crystals under the testing concentrations and it also promoted the nucleation rate of calcium oxalate crystals. Folium Pyrrosiae was shown to have an inhibitory effect on crystal growth rate and promoting nucleation only in high concentration (1.0 g/L), the change in nucleation and growth rate were not remarkable at lower concentrations (0.5 g/L and 0.1 g/L). The nucleation rate and growth rate of calcium oxalate crystals were measured from the crystal growth kinetic equation with input of the crystal size distribution acquired from the Coulter Multi-sizer. The results of the oxalate induced turbidity method showed that the nucleation rate of calcium oxalate crystals was found to be increased at all concentrations of Herb Lysimachiae and Folium Pyrrosiae. Increasing nucleation rate of calcium oxalate crystals can decrease the concentration of the salt in urine to a level below the supersaturation state and further crystals cannot form when the concentration of salt is below the supersaturation level. The smaller crystals formed can be passed out with urine easily as they do not get obstructed in the renal tubules, the crystals may get obstructed when their size is bigger. On the other hand, decreasing the growth rate of calcium oxalate crystals can prevent from the formation of crystals large enough to get obstructed in the renal tubules; crystals getting obstructed in the renal tubules can predispose to the formation of renal stone. The results showed that Herba Lysimachiae and Folium Pyrrosiae may be effective in the treatment or prevention of renal stone disease by increasing the nucleation rate and inhibiting the growth of calcium oxalate crystals.

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