Prevalence and antimicrobial susceptibility pattern of coagulase-positive staphylococci other than Staphylococcus aureus isolated from dogs and their owners

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Prevalence and antimicrobial susceptibility pattern of coagulase-positive staphylococci other than Staphylococcus aureus isolated from dogs and their owners

 

Author: Ko, Yuk-yu Denna
Title: Prevalence and antimicrobial susceptibility pattern of coagulase-positive staphylococci other than Staphylococcus aureus isolated from dogs and their owners
Degree: M.Sc.
Year: 2007
Subject: Hong Kong Polytechnic University -- Dissertations.
Veterinary medicine.
Staphylococcus aureus.
Staphylococcal infections.
Microbial sensitivity tests.
Department: Dept. of Health Technology and Informatics
Pages: iv, 69 leaves ; 30 cm.
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b2174231
URI: http://theses.lib.polyu.edu.hk/handle/200/2511
Abstract: Usage of antibiotics in treatment of pet animals has been increasing, the emergence and spread of antibiotic-resistant bacteria through pet animals to humans is of concern. As pet annuals are potential sources of transferring resistant bacteria to human, it is worthy to investigate the prevalence and susceptibility pattern of the isolates from animals. Aims: This project was undertaken: To determine the frequency of colonization of coagulase-positive Staphylococci other than Staphylococcus aureus isolated from dogs and owners; To investigate association between level of contact between dogs and owners, and colonization of the coagulase-positive Staphylococci; To determine rates of antibiotic resistance of coagulase-positive Staphylococci and compared the level of resistance in coagulase-positive Staphylococci isolated from dogs and humans. Methods and Result: Nasal swab samples from 815 dogs and 740 humans were collected from seven veterinary clinics in Hong Kong. All coagulase-positive staphylococci were identified by detecting the presence of catalase, coagulase, and p-galactosidase, production of acetoin, and fermentation of mannitol, trehalose, and xylose. A total of sixty-four (7.9%) dogs and eight (1.1%) humans carried Staphylococcus intermedius and one (0.1%) dog carried Staphylococcus schleiferi. Only four pairs of dogs and their owners carried S intermedius at the same time. Regard to the susceptibility to the antibiotics of the isolated S intermedius, 26.4% of strains were susceptible to all drugs tested including amoxycillin/ clavulanic acid, ampicillin, cefoxitin, cephalothin, chloramphenicol, ciprofloxacin, clindamycin, enrofloxacin, gentamicin, moxifloxacin, oxacillin, penicillin, tecicoplanin, tetracycline, trimethoprim-sulphamethoxazole, and vancomycm. The S schleiferi isolated was only resistance to amipcillin and penicillin. Increased risks of colonization of S intermedius were observed if dog were sleeping outside, suffering from chronic illness, at young age (<1 year old), or being owned from 1-12 months time. Conclusions: The low numbers of coagulase-positive staphylococci other than S. aureus isolated from humans samples failed to demonstrate the association between the level of contact between dogs and owners and colonization with S intermedius. No oxacillin-resistant S intermedius were recovered and amoxycillin/ clavulanic acid, cefoxitin, and cephalothin were found to be effective antimicrobial agents for treatment of S intermedium in dogs. But, the S intermedius isolates were highly resistant to clindamycin, trimethoprim-sulphamethoxazole, chloramphenicol, ciprofloxacin, enrofloxacin, moxifloxacin and tetracycline. Results indicated the over usage of antibiotics in veterinary and stringent control of the administration of antibiotics may be helpful in limiting the emergence of resistant S intermedius.

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