Comparative study of the antimicrobial activities of three antiseptics : a pilot study

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Comparative study of the antimicrobial activities of three antiseptics : a pilot study

 

Author: Wu, Sin-ying
Title: Comparative study of the antimicrobial activities of three antiseptics : a pilot study
Degree: M.Sc.
Year: 2007
Subject: Hong Kong Polytechnic University -- Dissertations.
Antiseptics.
Skin -- Physiological aspects.
Department: School of Nursing
Pages: xi, 147 leaves : ill. (some col.) ; 30 cm.
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b2115919
URI: http://theses.lib.polyu.edu.hk/handle/200/2677
Abstract: Background: Effective skin preparation is the most important step in preventing possible catheter-site infection, catheter-related systemic infection, and sample contamination. Purpose: The antimicrobial activities of all three testing antiseptics: 10% PVI, 0.5% CHG/ IPA and 2% TI, were evaluated in both one-step single antiseptics ( Al, Bl, Cl) and the two-step combined use of two antiseptics in the same skin antiseptic procedures (A2, B2, C2) at different contact time. Design: Quasi-experimental study Method: 13 participants was recruited from the convenience sampling method and participated to all skin antiseptic methods (Al, A2, Bl, B2, Cl and C2). For each participant, right cubital fossa of the participant was performed the culture test first for two-step skin antiseptic methods and then the left cubital fossa for the one-step skin antiseptic method. Skin swab was obtained for culture at each contact time after skin preparation to determine the microbial counts. Results: 0.5%CHG(IPA) (method Bl) required the shortest contact time at 1 minute to achieve the best antimicrobial activity within 2.5 minutes of contact time. 10% PVI (method Al), PVI/CHG(IPA) (Method B2) and 2% TI (Method Cl) required the longest contact time at 2.5 minutes. The forms of application did not different in the antimicrobial activities of the antiseptics. Among the three one-step skin antiseptic methods (PVI group, CHG group and TI group, skin antisepsis with 0.5%CHG(IPA) alone (method Bl) showed the lowest mean microbial counts, followed by 10% PVI (method Al) antisepsis and the highest mean microbial counts were found using 2% TI (method Cl) antisepsis at all contact times. The comparisons of the three two-step skin preparation methods revealed the better antimicrobial activity of 10% PVI than that of 2% TI with the same secondary antiseptics. The antisepsis first by PVI, followed by 70% IPA was more effective than that followed by 0.5%CHG/IPA in reduction of microbial counts in 30-seconds contact time. Using 10% PVI in one-step skin antiseptic preparation was equally effective when combined formulation, 2% TI, utilized in one-step method. Conclusions: Skin antisepsis with 0.5%CHG(IPA) in one-step skin preparation method was the best alternatives to the traditional two-step skin preparation method. The current practice including the two-step skin preparation method and choice of the antiseptics may need reexamination.

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