Establishment of an in-vitro artificial crystallizer to determine the effects of urinary macromolecules on the risks of renal stone diseases

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Establishment of an in-vitro artificial crystallizer to determine the effects of urinary macromolecules on the risks of renal stone diseases

 

Author: Chan, Yin-leung
Title: Establishment of an in-vitro artificial crystallizer to determine the effects of urinary macromolecules on the risks of renal stone diseases
Degree: M.Sc.
Year: 2001
Subject: Urine -- Analysis
Calcium oxalate
Hong Kong Polytechnic University -- Dissertations
Department: Multi-disciplinary Studies
Dept. of Nursing and Health Sciences
Pages: xi, 77 leaves : ill. ; 30 cm
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b1573253
URI: http://theses.lib.polyu.edu.hk/handle/200/2960
Abstract: Urinary macromolecules, originated from connective tissue, are thought to be involved in controlling crystal formation in the cases of urolithiasis. This work was aimed at studying the major family of this macromolecules found in urine, glycosaminoglycans(GAGs), that may control crystal formation from calcium and oxalate ions in the environment similar to renal milieu. To conduct the investigation of the effect of the glycosaminoglycans on the crystallization of calcium oxalate, a Mixed Suspension Mixed Product Removal (MSMPR) crystalliser was established. Two identical MSMPR chambers were set up to contain 20 mL of artificial urine with the other chamber serving as a control. By mean of this system, nucleation rate and growth rate of calcium oxalate crystals were determined by using the Coulter Multisizer counter. By introducing various concentrations of commercial GAGs, heparan sulphate(HS), dermatan sulphate(DS), chondroitin sulphate(CS) and hyaluronic acid(HA) into the test chamber, the effects of these components on crystallization of calcium oxalate in terms of nucleation and growth rate were then be evaluated. It was found that heparan sulphate enhanced nucleation rate and suppressed the growth rate on crystallization. Dermatan sulphate inhibited the growth of crystal but enhanced nucleation at low concentration. The chondrontin sulphate and hyaluronic acid gave contradictory results between low and high concentration. Chondrontin sulphate promoted growth at low concentration but not in high concentration. The reverse was the picture of hyaluronic acid, and at high concentration of hyaluronic acid, it suppressed the nucleation and promote the growth of calcium oxalate crystallization.

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