The diagnostic validity of serum alkaline phosphatase isoenzymes in patients with high alkaline phosphatase activity using isoelectric focusing technique

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The diagnostic validity of serum alkaline phosphatase isoenzymes in patients with high alkaline phosphatase activity using isoelectric focusing technique

 

Author: Tam, Chui-fong Yvonne
Title: The diagnostic validity of serum alkaline phosphatase isoenzymes in patients with high alkaline phosphatase activity using isoelectric focusing technique
Degree: M.Sc.
Year: 1999
Subject: Alkaline phosphatase
Isoenzymes
Isoelectric focusing
Hong Kong Polytechnic University -- Dissertations
Department: Multi-disciplinary Studies
Dept. of Nursing and Health Sciences
Pages: vi, 68 leaves : ill. ; 31 cm
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b1477302
URI: http://theses.lib.polyu.edu.hk/handle/200/354
Abstract: Fractionation of alkaline phosphatase isoenzymes and isoforms by isoelectric focusing was a simple procedure that up to 12 major bands and some sub-bands having ALP activity being resolved. The activity of the isoenzymes might reflect specific tissue injury. This method employed the commercially available Resolve(R) Alkaline Phosphatase test kit that was performed using Multiphor II electrophoresis system. The between batch coefficient of variations (CVs) for the assay was between 4.13 - 17.02 %. The data showed that with the separation procedure of IEF on agarose, all known isoenzymes were clearly identifiable. They were distributed in a limited number of sub-bands but this might be advantageous for diagnostic purposes. This procedure was fairly simple to perform, less time-consuming and was suitable for diagnostic biochemistry laboratories. The study had used the activity patterns of isoenzymes of ALP to detect different disease groups with high alkaline phosphatase level. ALP isoenzyme patterns were studied in four groups of patients: 51 with malignant disease, 31 with nonmalignant disease, 30 with liver disease and 6 with skeletal disease. It was found out that the ALP isoenzymes provided diagnostic information in patients with skeletal diseases. There was no significant difference in alkaline phosphatase isoenzymes among other patient groups. Band 5 showed consistent increases in patients with skeletal disease while band 2a, 2b and 5 also had good sensitivity and specificity in this group of disease. Analysis of ALP by isoelectric focusing deserved further evaluation, because it might facilitate the early detection of patients with skeletal disease among others with high ALP activity.

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