Use of tea tree oil as a disinfectant against Methicillin Resistant Staphylococcus aureus, Staphylococcus aureus and Escherichia coli

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Use of tea tree oil as a disinfectant against Methicillin Resistant Staphylococcus aureus, Staphylococcus aureus and Escherichia coli

 

Author: Wong, Yin-ling
Title: Use of tea tree oil as a disinfectant against Methicillin Resistant Staphylococcus aureus, Staphylococcus aureus and Escherichia coli
Degree: M.Sc.
Year: 2009
Subject: Hong Kong Polytechnic University -- Dissertations.
Tea tree oil.
Disinfection and disinfectants.
Methicillin resistance.
Department: Dept. of Health Technology and Informatics
Pages: vii, 58 leaves ; 30 cm.
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b2305531
URI: http://theses.lib.polyu.edu.hk/handle/200/4089
Abstract: Methicillin Resistant Staphylococcus aureus (MRSA) has been a serious public health hazard. Essential oils and other extracts of plants have evoked scientific interest as natural sources of antibacterial products. The purpose of this study was to examine the antibacterial effects of tea tree essential oil on MRSA, Staphylococcus aureus (SA) and Escherichia coli (E. coli) on plastic and metal surfaces. The antibacterial activity was evaluated by a standard quantitative carrier test method American Society for Testing and Materials (ASTM) with modification. 105 CFU/ml of the test organism were inoculated onto the carrier and air-dried, which was then transferred to the 1 ml of tea tree oil or Clorox(R) and treated for 30 min. After treatment, 1 ml disinfectant was neutralized with 9 ml normal saline. Then drawn out 0.lml and serial 10-fold dilutions were prepared. 100 ul of each serial dilution was spread onto nutrient agar surface. Finally, the plates were incubated for 24 hrs at 37oC. After incubation, colonies on agar plates were counted and log10 reductions in the viability titer of the test organism calculated. For the experiment on plastic surfaces, a 1% solution of tea tree essential oil significantly showed 100% reduction in E. coli and S.A., similar results were observed in 1% Clorox(R) For the MRSA testing, the effect of 1% tea tree oil (TTO) had been notified a reduction rate is 90% on plastic material. Moreover, 1% of tea tree essential oil significantly showed 100% reduction against E. coli and S.A. and with similar effect of Clorox(R) on metal surfaces. Last but not the least, the effect of 1% tea tree oil on metal surfaces demonstrated a promising reduction rate of 99.9% against MRSA. As observed in the study, a 1% solution of tea tree essential oil served as a disinfectant and demonstrated effective disinfection of plastic and metal surfaces in testing against MRSA, E. coli and S.A.

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