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DC FieldValueLanguage
dc.contributorDepartment of Health Technology and Informaticsen_US
dc.creatorWong, Yin-ling-
dc.identifier.urihttps://theses.lib.polyu.edu.hk/handle/200/4089-
dc.languageEnglishen_US
dc.publisherHong Kong Polytechnic University-
dc.rightsAll rights reserveden_US
dc.titleUse of tea tree oil as a disinfectant against Methicillin Resistant Staphylococcus aureus, Staphylococcus aureus and Escherichia colien_US
dcterms.abstractMethicillin Resistant Staphylococcus aureus (MRSA) has been a serious public health hazard. Essential oils and other extracts of plants have evoked scientific interest as natural sources of antibacterial products. The purpose of this study was to examine the antibacterial effects of tea tree essential oil on MRSA, Staphylococcus aureus (SA) and Escherichia coli (E. coli) on plastic and metal surfaces. The antibacterial activity was evaluated by a standard quantitative carrier test method American Society for Testing and Materials (ASTM) with modification. 105 CFU/ml of the test organism were inoculated onto the carrier and air-dried, which was then transferred to the 1 ml of tea tree oil or Clorox(R) and treated for 30 min. After treatment, 1 ml disinfectant was neutralized with 9 ml normal saline. Then drawn out 0.lml and serial 10-fold dilutions were prepared. 100 ul of each serial dilution was spread onto nutrient agar surface. Finally, the plates were incubated for 24 hrs at 37oC. After incubation, colonies on agar plates were counted and log10 reductions in the viability titer of the test organism calculated. For the experiment on plastic surfaces, a 1% solution of tea tree essential oil significantly showed 100% reduction in E. coli and S.A., similar results were observed in 1% Clorox(R) For the MRSA testing, the effect of 1% tea tree oil (TTO) had been notified a reduction rate is 90% on plastic material. Moreover, 1% of tea tree essential oil significantly showed 100% reduction against E. coli and S.A. and with similar effect of Clorox(R) on metal surfaces. Last but not the least, the effect of 1% tea tree oil on metal surfaces demonstrated a promising reduction rate of 99.9% against MRSA. As observed in the study, a 1% solution of tea tree essential oil served as a disinfectant and demonstrated effective disinfection of plastic and metal surfaces in testing against MRSA, E. coli and S.A.en_US
dcterms.extentvii, 58 leaves ; 30 cm.en_US
dcterms.isPartOfPolyU Electronic Thesesen_US
dcterms.issued2009en_US
dcterms.educationalLevelAll Masteren_US
dcterms.educationalLevelM.Sc.en_US
dcterms.LCSHHong Kong Polytechnic University -- Dissertations.en_US
dcterms.LCSHTea tree oil.en_US
dcterms.LCSHDisinfection and disinfectants.en_US
dcterms.LCSHMethicillin resistance.en_US
dcterms.accessRightsrestricted accessen_US

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Please use this identifier to cite or link to this item: https://theses.lib.polyu.edu.hk/handle/200/4089