Histochemical localization of constitutive and induced nitric oxide synthase in big head carp (Aristichthys nobilis) using NADPH-diaphorase activitystaining

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Histochemical localization of constitutive and induced nitric oxide synthase in big head carp (Aristichthys nobilis) using NADPH-diaphorase activitystaining

 

Author: Han, Kam-chu
Title: Histochemical localization of constitutive and induced nitric oxide synthase in big head carp (Aristichthys nobilis) using NADPH-diaphorase activitystaining
Degree: M.Sc.
Year: 1998
Subject: Nitric oxide
Nitric oxide -- Physiological effect
Fishes -- Physiology
Hong Kong Polytechnic University -- Dissertations
Department: Multi-disciplinary Studies
Dept. of Applied Biology and Chemical Technology
Pages: viii, 73 leaves : col. ill. ; 30 cm
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b1442183
URI: http://theses.lib.polyu.edu.hk/handle/200/4165
Abstract: Nitric Oxide Synthase (NOS) was found in many mammalian organs such as the brain, gut, liver, retina and endothelial cells. Recently, constitutive NOS was found in plant embryonic tissues, invertebrates as well as bacteria. However, in the fish, existence of NOS was only reported in the retina and branchial nerve. In this study, NADPH-diaphorase histochemical staining after paraformaldehyde fixation was used to demonstrate the existence of both constitutive and induced NOS activities in fresh frozen sections of organs and tissues of big head carp (Aristichthys nobilis). It was found that cNOS activity was strongest in neurons of the brain, and cells in the epithelium and mucosal layers in the intestines. Although not as strong as the cells in the brain and gut, the hepatic cells, renal tubular cells and ventricular cells of the heart also showed NOS activity. To a much less extent, muscle cells of the auricles as well as cells of the swimming bladder showed some NOS activity as well. For the iNOS activity induced by lipopolysaccharide (LPS), the staining intensity after challenged by LPS did not increase in brain, swim bladder, muscle and gall bladder. However, the epithelial cells of the intestine, hepatocytes of the liver and tubular cells of the kidney showed a great increase in staining intensity which suggested that iNOS was induced in these organs. Image analysis of the tissue sections showed that the percentage of cells showing NOS activity also increased in these organs.

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