| Author: | Man, Kwai-fun |
| Title: | A comparative study of the gel-centrifugation technique and flow cytometry for the detection of "PNH red cell" populations in the hematological disorders |
| Degree: | M.Sc. |
| Year: | 2000 |
| Subject: | Blood -- Diseases Erythrocytes Hong Kong Polytechnic University -- Dissertations |
| Department: | Multi-disciplinary Studies Department of Nursing and Health Sciences |
| Pages: | ix, 70 leaves : ill. (some col.) ; 30 cm |
| Language: | English |
| Abstract: | Paroxysmal Nocturnal Hemoglobinuria (PNH) is an acquired clonal disorder due to somatic mutations of the Phosphatidylinositol Glycan A (PIG-A) gene of the X-chromosome that leads to defective biosynthesis of the Glycosylphosphatidylinositol (GPI) anchor. It is characterized by an unusual sensitivity of abnormal red cell populations to complement lysis, due to a complete lack or partial deficiency of complement inhibitory surface GPI-linked protein molecules including CD55 and CD59. PNH has been associated with various hematological disorders. The demonstration of the abnormal PNH red cells can be illustrated by complement mediated lysis test or flow cytometry. In this study, a newly introduced method, the DiaMed-ID Microtyping System for PNH based on Gel-centrifugation Technique was used to detect the presence of CD55 or CD59 defective red cells populations in several hematological disorders including genuine PNH disease. 30 samples with 30 normal controls were analyzed by Sucrose Lysis test and Ham's test to screen for the presence of PNH defective red cells. The first ten samples together with the normal controls were analyzed by the DiaMed-ID Microtyping System to set up the baseline. Another ten samples which were particularly requested for Ham's test, were analyzed by the gel test as well. Due to resource limitation, as a rule, only positive results of gel test would be evaluated by flow cytometry. For the two PNH cases, the gel test results were directly compared with flow cytometric analysis and concordant conclusion was achieved. It was found that a very small population of CD55 or CD59 defective red cells was detected in a few cases by the DiaMed-ID Microtyping System but not by the flow cytometry. Defective red cells were detected in normal subjects as well. Though these findings need further investigations, nevertheless the DiaMed-ID Microtyping System for PNH although non-specific, seems to be an alternative method in screening the abnormal PNH red cell populations in genuine PNH as well as other hematological disorders. |
| Rights: | All rights reserved |
| Access: | restricted access |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| b1541792x.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 3.7 MB | Adobe PDF | View/Open |
Copyright Undertaking
As a bona fide Library user, I declare that:
- I will abide by the rules and legal ordinances governing copyright regarding the use of the Database.
- I will use the Database for the purpose of my research or private study only and not for circulation or further reproduction or any other purpose.
- I agree to indemnify and hold the University harmless from and against any loss, damage, cost, liability or expenses arising from copyright infringement or unauthorized usage.
By downloading any item(s) listed above, you acknowledge that you have read and understood the copyright undertaking as stated above, and agree to be bound by all of its terms.
Please use this identifier to cite or link to this item:
https://theses.lib.polyu.edu.hk/handle/200/4481