Construction of circular oligodeoxyribonucleotides on the structural basis of i-motif

Pao Yue-kong Library Electronic Theses Database

Construction of circular oligodeoxyribonucleotides on the structural basis of i-motif

 

Author: Liu, Dongsheng
Title: Construction of circular oligodeoxyribonucleotides on the structural basis of i-motif
Degree: Ph.D.
Year: 2002
Subject: Hong Kong Polytechnic University -- Dissertations
Oligonucleotides
Genetic regulation
Department: Dept. of Applied Biology and Chemical Technology
Pages: xvi, 118 leaves : ill. ; 30 cm
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b1641628
URI: http://theses.lib.polyu.edu.hk/handle/200/5320
Abstract: As illustrated in Figure A-1, it was discovered for the first time that beyond the scope of the duplex and triplex strategies, the i-motif, a four stranded assembly based on C+.C base pairs formed under a slightly acidic condition, can direct the sequence-specific formation of a phosphodiester linkage between 3' hydroxyl group and 5' phosphate group of linear oligodeoxyribonucleotides with high efficiency and high sequence-selectivity by chemical activation and thus represents a new type of structural template for constructing circular oligodeoxyribonucleotides. Based on this new strategy, a 28-mer circular oligodeoxyribonucleotide has been successfully constructed on intramolecular i-motif structure, exonuclease VII, exonuclease I and Alkaline Phosphatase digestion have confirmed the circular nature of this product. Furthermore, i-motif structure has been demonstrated as the real intermediate of the circularization process via studying the relation between yield of circular products and factors influencing the i-motif structure formation. Furthermore, when this conception was applied to the bimolecular i-motif structure, circular oligodeoxyribonucleotide possessing as few as 9 nucleotides, the smallest template-directed synthetic oligodeoxyribonucleotide achieved up to now, has been prepared. In conclusion, utilization of the unique self-recognition pattern of the i-motif in the current study not only represents a distinctive strategy for constructing circular oligonucleotides but also opens up a new method for the synthesis of oligonucleotide sequences which are not accessible via other methodologies.

Files in this item

Files Size Format
b16416284.pdf 5.109Mb PDF
Copyright Undertaking
As a bona fide Library user, I declare that:
  1. I will abide by the rules and legal ordinances governing copyright regarding the use of the Database.
  2. I will use the Database for the purpose of my research or private study only and not for circulation or further reproduction or any other purpose.
  3. I agree to indemnify and hold the University harmless from and against any loss, damage, cost, liability or expenses arising from copyright infringement or unauthorized usage.
By downloading any item(s) listed above, you acknowledge that you have read and understood the copyright undertaking as stated above, and agree to be bound by all of its terms.

     

Quick Search

Browse

More Information