Fabrication of microengineered polyethylene glycol (PEG) hydrogel for cell alignment and elongation

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Fabrication of microengineered polyethylene glycol (PEG) hydrogel for cell alignment and elongation

 

Author: Jing, di
Title: Fabrication of microengineered polyethylene glycol (PEG) hydrogel for cell alignment and elongation
Degree: M.Sc.
Year: 2010
Subject: Hong Kong Polytechnic University -- Dissertations
Polyethylene glycol.
Peptides
Tissue engineering
Bones -- Cytology.
Department: Dept. of Health Technology and Informatics
Pages: xii, 49 leaves : ill. ; 30 cm.
InnoPac Record: http://library.polyu.edu.hk/record=b2356845
URI: http://theses.lib.polyu.edu.hk/handle/200/5689
Abstract: In tissue engineering, some crucially important functional properties are directly to the cell behaviors such as the cellular aggregation, orientation and elongation. Therefore, the tissue engineering development critically depends on the modern knowledge of the guidance cues to cell culture. The objective of this research was to determine Arg-Gly-Asp (RGD) tripeptide on PEG hydrogel surface contact guidance on aggregation of osteoblasts and orientation and elongation of fibroblasts. After 24 hour culture, rat osteoblasts aggregating on RGD pattern surfaces were much more than those on the PEG surfaces. The surface with 70μm RGD pattern, statistically exhibited the strongest effect on rat osteoblasts aggregation than the 60 μm RGD pattern and pure PEG surfaces. The cells cultivated on 70μm RGD pattern were 130 times of them on PEG surface. But the cells cultivated on 60μm RGD pattern were 115 times of them on PEG surface too. Human foreskin fibroblasts response to RGD with different width scopes (50-70μm, 90-100μm) was qualitatively and quantitatively assessed by evaluating fibroblast orientation and elongation, compared to cells seeded on PEG hydrogels with uniform distribution of RGD peptides. Results analyzed by one-way ANOVA and post test suggest that fibroblasts can recognize the RGD pattern and orientate towards the RGD length. Statistically, the cells on 50 μm RGD pattern performed the greatest orientation along the RGD (P value <0.05), exhibiting the average orientation angle with 7.62°. When the RGD pattern narrower than 100 μm, it would show good fibrolasts orientation angle within 26°, compared the uniform distribution of RGD the mean orientation angle on it was 54°(P value <0.05), that is, the fibroblasts cultivated on RGD pattern would be growing in line along the long side of RGD pattern and the narrower, the parallel growing would be. The elongation data were collected by the ratio between the long and short axis of fibroblast. And the ratio value larger, the elongation is better. Results analyzed by one-way ANOVA and post test suggested that fibroblast cultivated on 50 μm RGD pattern expressed a significant difference against 100 μm RGD pattern (P<0.05), but there was no significant difference among the other groups (P>0.05). Overall, from the statistical Mean+SD figure, there is still a tendency that with the RGD pattern narrower (within 90μm), the elongation would be better.

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