Comparison of the incidence of chromosome 4q deletion between Chinese multiple myeloma patients and Caucasian multiple myeloma patients by using interphase flurorescent in situ hybridization (FISH)

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Comparison of the incidence of chromosome 4q deletion between Chinese multiple myeloma patients and Caucasian multiple myeloma patients by using interphase flurorescent in situ hybridization (FISH)

 

Author: Lam, Lai-ying
Title: Comparison of the incidence of chromosome 4q deletion between Chinese multiple myeloma patients and Caucasian multiple myeloma patients by using interphase flurorescent in situ hybridization (FISH)
Degree: M.Sc.
Year: 2010
Subject: Hong Kong Polytechnic University -- Dissertations
Multiple myeloma
Human chromosome abnormalities.
Fluorescence in situ hybridization
Department: Dept. of Health Technology and Informatics
Pages: x, 57 leaves : col. ill. ; 31 cm.
InnoPac Record: http://library.polyu.edu.hk/record=b2355949
URI: http://theses.lib.polyu.edu.hk/handle/200/5822
Abstract: Multiple myeloma (MM) is a malignant plasma cell disorder that normal plasma cells transform into malignant cells. It accounts for 10% of all haematologic malignancies in Caucasians and 20% in African Americans whereas Asians have the lowest incidence rate. From the previous study done in our laboratory, high frequency (42% -46%) of chromosome 4q deletion were first discovered in the Chinese ethnic group by using fluorescence in situ hybridization (FISH) on bone marrow aspirates. FISH is widely used to study numerical and structural genetic abnormalities in both metaphase and interphase cells. It provides researchers with a way to visualize and map the genetic materials in an individual's cells, including specific genes or portions of genes. This study was the continuity of the previous study but using paraffin trephine section instead of bone marrow aspirates. For multiple myeloma, there are only a few studies using FISH technique on paraffin trephine sections due to the difficult optimization condition. In this study, a protocol for FISH on archival samples was established. By looking for fern like structures under light microscope after different proteinase K digestion time intervals, one and a half hours was the most optimal time for the enzyme digestion. By using paraffin trephine section, the frequency of chromosome 4q deletion among the 30 Chinese MM patients was 40% and it was comparable to the previous study. Moreover, there was no relationship between different laboratory data and the deletion status of chromosome 4q deletion (p-value: <0.05) except the plasma cell percentage in bone marrow and this would need to be further investigated in the future. For the 34 Caucasian MM patients, due to the quality of the slides, only 15 samples could be counted and only 1 of the 15 samples could be counted for 100 plasma cells. The frequency of chromosome 4q deletion was 27%. The difference in the frequency of chromosome 4q deletion among Chinese and Caucasian ethnic group was statistically insignificant (p=0.38). However, the sample size of this study was too small and the data showed that there was a trend that the chromosome 4q deletion was more frequent among the Chinese MM patients. To further study on this issue, more samples should be recruited in order to investigate the frequency of chromosome 4q deletion among different ethnic group and hence for comparisons. It will help to give a clearer picture on the genetic events of multiple myeloma tumoreigenesis.

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