The effect of Huachansu injection as an anti-cancer immunomodulatory traditional Chinese medicine on blood dendritic cells

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The effect of Huachansu injection as an anti-cancer immunomodulatory traditional Chinese medicine on blood dendritic cells

 

Author: Ng, Wai-chung
Title: The effect of Huachansu injection as an anti-cancer immunomodulatory traditional Chinese medicine on blood dendritic cells
Degree: M.Sc.
Year: 2011
Subject: Medicine, Chinese.
Dendritic cells.
Dendritic cells -- Immunology.
Cancer -- Treatment.
Hong Kong Polytechnic University -- Dissertations
Department: Dept. of Health Technology and Informatics
Pages: xii, 74 leaves : ill. (some col.) ; 30 cm.
InnoPac Record: http://library.polyu.edu.hk/record=b2451604
URI: http://theses.lib.polyu.edu.hk/handle/200/6148
Abstract: Dendritic cells (DCs) are professional antigen presenting cells that can prime naive T-cells linking both the innate and adaptive parts of the immune system. Basically, there are two major subsets which are Myeloid Dendritic Cells (MDCs) and Plasmacytoid Dendritic cells (PDCs). MDC could be phenotypically defined as Lin⁻ HLA-DR⁺ CD11c⁺ CD123⁻ whereas PDC by Lin⁻ HLA-DR⁺ CD11c⁻ CD123⁺ . The objective of the study was to investigate the effect of Huachansu injection as an anti-cancer immunomodulatory traditional Chinese medicine on blood DCs. It was hypothesized that the Huachansu injection might quantitatively or functionally change the subsets of DCs to inhibit tumour growth. By measuring the expression of maturation markers CD83, co-stimulatory molecules CD80 and Major Histocompatibility Complex (MHC) Class II molecules (HLA-DR) on DCs, the mechanism of Huachansu action would be deciphered. Our study demonstrated that the number of MDC was significantly decreased when compared with untreated control (p = 0.0006) whereas the number of PDC was found to increase but was not statistical significant (p = 0.2250). On the other hand, both the Mean Fluorescence Intensity (MFI) for CD11c⁺ DC (MDC) (p = 0.1224) and that for CD123⁺ DC (PDC) (p = 0.6780) were not significantly increased comparing to untreated control although reflected an increasing trend after 24 hours of incubation with Huachansu injection. The Mean Fluorescence Intensity (MFI) for CD80⁺ CD83⁺ DC (p = 0.0187) was significantly decreased compared with untreated control whereas that for CD80⁺ CD83⁻ DC demonstrated contrasting statistical concluded results for the day 1 (p = 0.0139) and day 2 observation (p=0.3830) with increasing trend. The Mean Fluorescence Intensity (MFI) for HLA-DR⁺ DC (p = 0.2050) demonstrated an increasing tendency but was not statistical significant. The number of CD80⁺CD83⁺ DC (p = 0.3010) was found to be increased while that for CD80⁺CD83⁻ DC (p = 0.2838) demonstrated a decrease trend but was not statistical significant. It was concluded that Huachansu injection might significantly reduce the number of MDCs and down-regulate the expression of CD80⁺CD83⁺DCs.

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