Effect of GPMS, APTES, and TPM surface treatment on nanoporous AAO membrane to immobilize E. coli and staphylococcus antibodies for enhancing cellular attachment

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Effect of GPMS, APTES, and TPM surface treatment on nanoporous AAO membrane to immobilize E. coli and staphylococcus antibodies for enhancing cellular attachment

 

Author: Cheung, Kai-wing Kelvin
Title: Effect of GPMS, APTES, and TPM surface treatment on nanoporous AAO membrane to immobilize E. coli and staphylococcus antibodies for enhancing cellular attachment
Degree: M.Sc.
Year: 2011
Subject: Biosensors.
Nanostructured materials.
Surfaces (Technology) -- Analysis.
Hong Kong Polytechnic University -- Dissertations
Department: Dept. of Health Technology and Informatics
Pages: 72 leaves : ill. ; 30 cm.
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b2462945
URI: http://theses.lib.polyu.edu.hk/handle/200/6330
Abstract: In nowadays research industry, such as gene mapping, clinical diagnostics, and drugs delivery, requires efficient sensing and monitoring of biological molecules such as proteins, enzymes, and DNA. It is crucial to have an efficient and cost-effective biosensing platform. Nanoporous AAO membrane has been a prevalent platform as the large internal surface area facilitates high throughput and sensitive biosensing. It is important that the surface of the designated platform has the ability to immobilize biomolecules for enhanced substrate attachment. AAO membrane surface itself does not provide proficient bonding of substrate molecules. In order to enhance the surface attachment of AAO membranes, GPMS, APTES, and TPM treatments are utilized in this study to functionalize the surface properties. E. Coli and Staphylococcus antibodies are then implemented respectively on different treated membranes to observe the level of bonding.

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