| Author: | Cheung, Kai-wing Kelvin |
| Title: | Effect of GPMS, APTES, and TPM surface treatment on nanoporous AAO membrane to immobilize E. coli and staphylococcus antibodies for enhancing cellular attachment |
| Degree: | M.Sc. |
| Year: | 2011 |
| Subject: | Biosensors. Nanostructured materials. Surfaces (Technology) -- Analysis. Hong Kong Polytechnic University -- Dissertations |
| Department: | Department of Health Technology and Informatics |
| Pages: | 72 leaves : ill. ; 30 cm. |
| Language: | English |
| Abstract: | In nowadays research industry, such as gene mapping, clinical diagnostics, and drugs delivery, requires efficient sensing and monitoring of biological molecules such as proteins, enzymes, and DNA. It is crucial to have an efficient and cost-effective biosensing platform. Nanoporous AAO membrane has been a prevalent platform as the large internal surface area facilitates high throughput and sensitive biosensing. It is important that the surface of the designated platform has the ability to immobilize biomolecules for enhanced substrate attachment. AAO membrane surface itself does not provide proficient bonding of substrate molecules. In order to enhance the surface attachment of AAO membranes, GPMS, APTES, and TPM treatments are utilized in this study to functionalize the surface properties. E. Coli and Staphylococcus antibodies are then implemented respectively on different treated membranes to observe the level of bonding. |
| Rights: | All rights reserved |
| Access: | restricted access |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| b24629455.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 5.64 MB | Adobe PDF | View/Open |
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