The study of cancer stem cells in triple negative subtype of human ductal carcinoma

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The study of cancer stem cells in triple negative subtype of human ductal carcinoma

 

Author: Yu, Wan-seung
Title: The study of cancer stem cells in triple negative subtype of human ductal carcinoma
Degree: M.Sc.
Year: 2012
Subject: Breast -- Cancer -- Molecular aspects.
Cancer cells.
Stem cells.
Hong Kong Polytechnic University -- Dissertations
Department: Dept. of Health Technology and Informatics
Pages: xxiii, 168 leaves : col. ill. ; 30 cm.
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b2475278
URI: http://theses.lib.polyu.edu.hk/handle/200/6443
Abstract: Triple negative (TN) breast cancer is an aggressive subtype and frequently occurs in females of younger age, Hispanic ethnic group and BRCA1 mutation. It has worse prognosis due to its insensitivity to radio-and hormonal therapies, and high recurrence. Nowadays cancer stem cells (CSCs) are thought to play role in poor prognosis. They were defined as rare populations of cancer cells possessing self-renewal capacity and radio- and/ chemo-resistance to conventional cancer treatment. As this theory is proven, it is critical to identify and subsequent eradicate them in patient management. Our study focuses on CSCs in TN subtype of ductal carcinoma. We study three particular cancer subsets with phenotype of: CD44⁺CD24⁻/low, CD44⁺CD24⁺ and CD44⁻CD24⁺ using immunohistochemistry. The aim is to determine if three subsets could be putative CSCs in TN cancer. These findings demonstrate significance in three aspects: discovery of novel prognostic markers, potential therapeutic target and development of better strategies for treatment in TN patients. Double-staining technique was applied to demonstrate expression of CD44 and CD24 marker. Prevalence of the expression in each subset was estimated and was then correlated with clinico-pathological data. Immunohistochemistry results showed that the most prevalence subsets in human TN tissue is the CD44⁺CD24⁻/low population, which constitutes 42.3% of all 26 cases. In addition, its expression is significantly higher in tumor grade III than grade II (P=0.046<0.05, 2-tailed). In conclusion, this study shows that CD44⁺CD24⁻/low is the most predominant CSC phenotypes in TN subtype of ductal carcinoma and its upsurge in grade III that shed light on CSC-based target therapy.

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