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dc.contributorDepartment of Health Technology and Informaticsen_US
dc.creatorChan, Chun Yu Ron-
dc.identifier.urihttps://theses.lib.polyu.edu.hk/handle/200/6557-
dc.languageEnglishen_US
dc.publisherHong Kong Polytechnic University-
dc.rightsAll rights reserveden_US
dc.titlePolyethylene-glycol hydrogel scaffold conjugated with cell adhesion peptide for cell cultureen_US
dcterms.abstractResults of nerve damage various; including loss of sensation, motor movement impairment, which depends on the site of incision. Traditionally, nerve severance was treated by autograph, requiring the surgeon to extract another segment of nerve from the patient. This will create another opening wound on the patient and the healing of nerve may not be satisfying. Tissue engineering is a very hot research topic providing new cues in treating nerve severance. Nerve guidance conduit, which is basically a cell culture scaffold, could help to guide and stimulate nerve regeneration. Poly (ethylene-glycol) hydrogel, which is highly hydrophilic, biocompatible, non-toxic and wildly applied in medical treatment, was considered as a potential material for fabricating nerve guidance conduit. It is often described as a "blank slate", while properties can be "written" on it. The tunable characteristic made it very suitable for being a nerve guidance conduit. In this study, cell adhesion peptide, a component in extracellular matrix, was immobilized in poly (ethylene-glycol). The cell adhesion peptide provided binding site for integrin receptors on cell surface, allowing cells to bind on a hydrophilic hydrogel. Experimental result demonstrated that when the cell adhesion peptide concentration in hydrogel reached 5.3μmol/cm³, neurite extension activity was found on cells seeded on it. This exploratory study verified the capability of cell adhesion peptide modified four-arm poly (ethylene-glycol) hydrogel of being a cell culturing scaffold which can support neurite extension. In future, it could become a wildly used tissue engineering scaffold thanks for its biocompatibility.en_US
dcterms.extentxiv, 50 leaves : ill. (some col.) ; 30 cm.en_US
dcterms.isPartOfPolyU Electronic Thesesen_US
dcterms.issued2012en_US
dcterms.educationalLevelAll Masteren_US
dcterms.educationalLevelM.Sc.en_US
dcterms.LCSHPolyethylene glycol.en_US
dcterms.LCSHCell adhesion.en_US
dcterms.LCSHHong Kong Polytechnic University -- Dissertationsen_US
dcterms.accessRightsrestricted accessen_US

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