Proteomic approach in the screening of immunomodulatory activities in traditional Chinese medicine

Pao Yue-kong Library Electronic Theses Database

Proteomic approach in the screening of immunomodulatory activities in traditional Chinese medicine

 

Author: Wang, Yuen-yuen
Title: Proteomic approach in the screening of immunomodulatory activities in traditional Chinese medicine
Degree: M.Phil.
Year: 2002
Subject: Hong Kong Polytechnic University -- Dissertations.
Medicine, Chinese.
Herbs -- Therapeutic use.
Immunological adjuvants.
Department: Dept. of Applied Biology and Chemical Technology
Pages: xiii, 187 leaves : ill. ; 30 cm.
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b1641663
URI: http://theses.lib.polyu.edu.hk/handle/200/685
Abstract: The proteomic approach has fast evolved as the method of choice for novel drug discovery. Today, many screening programs with this approach use transformed cell lines as the effectors for investigation. After exposing to the compound of interest, enhanced propagation of a cell line will denote prospective immunomodulatory activities. However, this "popular." approach does not account for the fact that the induction of a wide spectrum of immunomodulatory activities requires the presence and interactions of various types of immune cells including macrophages, B cells and T cells. More importantly, using this approach, multi-targeting effects will be difficult to detect. In our study, we used primary splenocytes as the effectors. Splenocytes (which consists of macrophages, B- and T-cells found in the spleen) are capable of inducing a full spectrum of immunomodulatory activities. On the other hand, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) can be used to examine the concurrent expression of more than 1,000 different protein species. Therefore, multi-targeting effects of any drug under investigation through the up- or down-regulation of compounds can be detected conveniently. After confirming the immunomodulatory activities of ginsenoside Rgl on production of TNF-a and IFN-g using western blot and ELISA assay, comparison of 2D gel patterns between normal and Rgl treated samples was performed. Our results showed that 87 proteins were up-regulated, 38 were down-regulated, and 38 had no significant change. With the aid of MALDI-TOF-MS, 7 proteins were identified. For the identified proteins, the T cell surface glycoprotein CD5, DNA polymerase and homeotic protein HL-2 were up-regulated, and they are involved in the lymphocyte proliferation process. Cytochrome C oxidase and G protein are the other two identified up-regulated proteins, which are involved in the transmembrane signaling process and cytokine IL-2 induction process. Hypothetical anti-proliferative protein is the only identified protein that was down-regulated. It may be involved in the suppression of white cell proliferation. The role of another identified up-regulated protein, alpha-mannosidase II is not clear. The significance of this finding is currently unknown. Nevertheless, the exact immunomodulatory mechanism of Rgl can be easily demonstrated using this approach. Results of this study not only will be very useful in understanding immunomodulatory mechanisms of Rgl, this screening platform can also provide a framework and background for large-scale screening of TCM for immunomodulation as well as development of new drugs for cancer prevention and treatment.

Files in this item

Files Size Format
b16416636.pdf 15.18Mb PDF
Copyright Undertaking
As a bona fide Library user, I declare that:
  1. I will abide by the rules and legal ordinances governing copyright regarding the use of the Database.
  2. I will use the Database for the purpose of my research or private study only and not for circulation or further reproduction or any other purpose.
  3. I agree to indemnify and hold the University harmless from and against any loss, damage, cost, liability or expenses arising from copyright infringement or unauthorized usage.
By downloading any item(s) listed above, you acknowledge that you have read and understood the copyright undertaking as stated above, and agree to be bound by all of its terms.

     

Quick Search

Browse

More Information