Folic acid and fitc conjugated superparamagnetic nanoparticles for targeting MCF-7 cancer cells

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Folic acid and fitc conjugated superparamagnetic nanoparticles for targeting MCF-7 cancer cells


Author: Chen, Ao
Title: Folic acid and fitc conjugated superparamagnetic nanoparticles for targeting MCF-7 cancer cells
Degree: M.Sc.
Year: 2013
Subject: Cancer cells -- Identification.
Molecular Imaging.
Hong Kong Polytechnic University -- Dissertations
Department: Interdisciplinary Division of Biomedical Engineering
Pages: xiii, 51 leaves : ill. (some col.) ; 30 cm.
Language: English
Abstract: Currently, cancer is treated by radiologic or chemical therapy, shrinking the tumor and followed by surgical resection to remove the whole tissue. If the malignant tissue was not completely removed, metastasis of cancer should happen in high possibility. The goal of this research is to develop a theranostic nanoparticle, not only serves as a targeting probe for doctors during surgery, but also has the potential to treat cancer. Nano scale theranostic is a very hot research topic providing versatile platform in treating cancer. In this study, super paramagnetic iron oxide nanoparticle was synthesized by a facile hydrothermal method. The produced nanoparticle was highly hydrophobic and stable. Further characterization by transmission electron microscopy showed that the nanoparticle was well crystallized and had a narrow size distribution around 24 nm in diameter. A wide range of cancers could be potentially targeted by folic acid because numerous human cancer cells have been found to overexpress the folate receptor. In this study, folic acid and fluorescein isothiocyanate was functionalized with (3-aminopropyl)-triethoxysilane and conjugated to nanoparticle by silanization. The modified nanoparticles were characterized by Fourier transform infrared spectroscopy. Human breast carcinoma cell (MCF-7) was known to overexpress folate receptor and used in this study to examine the cellular uptake of folic acid conjugated nanoparticle. Different time interval was set to study the curve of cellular uptake. Fluorescent image was taken and quantified by ImageJ software. Data was analyzed by Microsoft Office Excel and IBM SPSS. It revealed that nanoparticle uptake increased as the incubation time prolonged. MCF-7 incubated with folic acid conjugated nanoparticles showed significantly higher nanoparticle uptake than folate free nanoparticles (P < 0.05). In addition, 90% of the cells had uptaken folic acid conjugated nanoparticle after 8 hours of incubation. In future, it could become a wildly used theranostic nanoparticle thanks to its facile synthesis, efficient targeting and high biocompatibility.

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