DNA absorption and desorption with functionalized nanoporous anodic aluminum oxide membrane

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DNA absorption and desorption with functionalized nanoporous anodic aluminum oxide membrane

 

Author: Yang, Suhan
Title: DNA absorption and desorption with functionalized nanoporous anodic aluminum oxide membrane
Degree: M.Sc.
Year: 2013
Subject: Mobile genetic elements.
DNA -- drug effects.
Hong Kong Polytechnic University -- Dissertations
Department: Interdisciplinary Division of Biomedical Engineering
Pages: ix, 49 leaves : ill. (some col.) ; 30 cm.
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b2558379
URI: http://theses.lib.polyu.edu.hk/handle/200/6872
Abstract: DNA extraction and purification from vivo sample is demanded in various research fields for genetic analysis process. Characterized by its chemical stability, potentials in diversified surface modification options and simplified protocols, solid-phase based method for DNA molecules isolation has widely discussed. In this project, a novel biomembrane: nanoporous anodic aluminum oxide (AAO) membrane was employed as DNA extraction substrate after APTES modification to investigate its optimal interaction conditions and to exam the different Lambda DNA binding capacities among three pore sizes. The best absorption and desorption conditions were pH 4.5 and pH 10.5, under these reaction conditions, Lambda DNA capturing ability increased with the amino group intensity.

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