The effect of Huachansu on human natural killer cells

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The effect of Huachansu on human natural killer cells

 

Author: Li, Shuk Yee
Title: The effect of Huachansu on human natural killer cells
Degree: M.Sc.
Year: 2013
Subject: Medicine, Chinese.
Killer cells.
Natural immunity.
Hong Kong Polytechnic University -- Dissertations
Department: Dept. of Health Technology and Informatics
Pages: xii, 73 leaves : ill. (some col.) ; 30 cm.
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b2637244
URI: http://theses.lib.polyu.edu.hk/handle/200/7069
Abstract: Huachansu (HCS) is an anti-tumor Traditional Chinese Medicine and widely used in China. HCS mainly composed of two biologically active chemical components: indole alkaloids and steroidal cardiac glycosides. Bufalin, resibufogenin and cinobufagin are the major components of steroidal cardiac glycosides and recent studies have shown that the antitumor activity of HCS is attributed to these three components. Natural Killer (NK) cells are important innate immune effector cells which can lyse the target cells without previous sensitization. NK cells also can produce cytokines and chemokines. Different subset of NK cells can be divided depend on their phenotype characteristic. In this dissertation, the effect of HCS on human blood Natural Killer cells was investigated. The absolute count and Mean Fluorescence Intensity (MFI) of different NK subsets on different HCS dilutions would be the investigated target. Five normal blood samples were used and 1/20 to 1/4860 HCS dilutions were chosen in this study. The major NK subset CD56dimCD16pos (90% of NK cell) had shown with the greatest effect on 1/20 dilution. Obviously functional effect might be seen even in small change of this NK subset. Studies had proven that CD56dimCD16pos NK subset had the highest cytolytic function. The absolute count of this subset was increased by 15% in 1/20 HCS dilution on Day 1. Besides, the absolute count of CD56dimCD16posCD8apos NK subset on both Day 1 and Day 2 were increased by 43% and 45% respectively. This might indicate the effect of HCS might be a long lasting effect on NK cells. The CD8apos MFI of this subset also changed. On Day 1 and Day 2, there were increased by 19% and 27% respectively in CD8apos MFI. It is assumed that the higher MFI on CD8apos, the higher cytolytic functions. Conclusion: HCS would enhance the immune system by increasing the absolute count and MFI of particular NK subset.

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