Graphene oxide based on fluorescence resonance energy transfer (FREF) biosensor for H5N1 DNA detection

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Graphene oxide based on fluorescence resonance energy transfer (FREF) biosensor for H5N1 DNA detection

 

Author: Meng, Nuo Ya
Title: Graphene oxide based on fluorescence resonance energy transfer (FREF) biosensor for H5N1 DNA detection
Degree: M.Sc.
Year: 2014
Subject: Avian influenza
Avian influenza -- Diagnosis.
Hong Kong Polytechnic University -- Dissertations
Department: Interdisciplinary Division of Biomedical Engineering
Pages: 58 leaves : illustrations (chiefly color) ; 30 cm
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b2757739
URI: http://theses.lib.polyu.edu.hk/handle/200/7554
Abstract: Human inflection with H5N1 avian flu has been found since 1997 and over 500 people infected by this virus. It is urgent to find a rapid and novel method to detect H5N1 virus with high efficiency and sensitivity. Based on absorption of graphene oxide (GO) with single-stranded DNA (ssDNA), a FRET biosensor based on FREF for H5N1 virus DNA has been developed. In our study, the H5N1 virus DNA was binding with fluorescent dye 5′6-FAM (5′6-carboxyfluorescein) as the source of fluorescence. When adding graphene oxide aqueous solution, H5N1 virus DNA was adsorbed to graphene oxide surface and the fluorescence of dye was quenched by graphene oxide. Subsequently, a short complementary DNA probe was utilized to specifically hybrid with H5N1 virus DNA, which leads the fluorescent dye FAM with double stranded DNA (dsDNA) released from graphene oxide surface, which results in recovery of the fluorescence. Experiment with non-complimentary DNA was explored for testify the specificity. The limit of detection of this GO based FRET biosensor is around 0.015uM. These results suggest the novel GO based FRET biosensor supplies a rapid approach for early H5N1 virus DNA diagnosis with high sensitivity and specificity.

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