Correlation study of the phytochemical composition and the neuroprotective bioactivity of Gastrodia elata from different sources

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Correlation study of the phytochemical composition and the neuroprotective bioactivity of Gastrodia elata from different sources

 

Author: Tang, Yat Man James
Title: Correlation study of the phytochemical composition and the neuroprotective bioactivity of Gastrodia elata from different sources
Degree: M.Sc.
Year: 2015
Subject: Gastrodia elata.
Hong Kong Polytechnic University -- Dissertations
Department: Dept. of Health Technology and Informatics
Pages: xvi, 125 leaves : illustraions ; 30 cm
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b2762050
URI: http://theses.lib.polyu.edu.hk/handle/200/7807
Abstract: Aim: The aim of this thesis is to investigate the correlation between the phytochemical composition and the neuroprotective bioactivity of Gastrodia elata (GE). Methods: Two approaches were used to investigate this correlation. Firstly, the biological approach is the neuroprotective bioactivity of GE extracts from four different sources (Anhui. Sichuan, Wupei, Yunnan) and five pure compounds of GE individually (GAS, 4-HBA, 4-HA, VA, VL) were determined by an in vitro Parkinson’s Disease (PD) model which is created by BE(2)-C cells subjected to 6-hydrodopamine (6-OHDA) neurotoxin insult. Secondly, the chemical approach is to determine the concentration of the five neuroprotective compounds in GE extracts of different sources by High Pressure Liquid Chromatography -Diode Array Detector (HPLC-DAD). The results from these two approaches were evaluated with Pearson product-moment correlation to determine if the concentrations of the five compounds in those various extracts would correlate with the coresponding neuroprotective bioactivity of individual GE extracts. Results: GE from all four sources had neuroprotective effect on BE(2)-C cells against 6-OHDA insult. The neuroprotective power (NP) of GE extracts were different (Anhui Sichuan; Anhui Yunnan) and the concentration of the five neuroprotective compounds were also highly variable. However, there was no statistically significant correlation (P > 0.05) between the concentrations of the five compounds in the GE extracts and their respective neuroprotective bioactivities. Conclusion: This study has used in vitro model using BE(2)-C cells subjected to 6-OHDA that was applicable for studying the neuroprotective bioactivity of GE. GE extracts from all four sources were exhibited neuroprotective effects, but their neuroprotective power varied. Furthermore, it was found that the neuroprotective bioactivity of individual GE extracts were not correlated to the concentrations of the five compounds of the respective GE extracts. This finding suggested that the quality control for GE requires more comprehensive approach, where the use of measuring a single compound of GAS alone was not sufficient to determine the quality of GE in relation to its correlating neuroprotective bioactivity.

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