Association between plasma leptin, adiponectin and leptin/adiponectin ratio with metabolic syndrome in adult Chinese population

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Association between plasma leptin, adiponectin and leptin/adiponectin ratio with metabolic syndrome in adult Chinese population

 

Author: Hui, Yat Hang Alex
Title: Association between plasma leptin, adiponectin and leptin/adiponectin ratio with metabolic syndrome in adult Chinese population
Degree: M.Sc.
Year: 2016
Subject: Metabolic Syndrome.
Leptin.
Fat cells.
Hong Kong Polytechnic University -- Dissertations
Department: Dept. of Health Technology and Informatics
Pages: 87 pages : illustrations
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b2925463
URI: http://theses.lib.polyu.edu.hk/handle/200/8715
Abstract: Metabolic Syndrome (MetS) is a major and rapidly growing public health problem in developed countries. The prevalence of MetS in Hong Kong is found increased from about 10-15% at 2005 to about 14 to 17% nowadays. Epidemiological studies revealed that MetS increase 5-fold risk of Type 2 DM, increase 2-fold risk of CVD, increase 2 to 4-fold risk of stroke and 3 to 4-fold risk of myocardial infarction (MI). Development of MetS is multifactorial including environmental and genetic factors. MetS composed of several cardiovascular and metabolic risks including central obesity (adiposity), glucose intolerance together with insulin resistance, dyslipidemia and hypertension. Other modifiable risk factors and biomarkers can early be identified to avoid further development of more adverse irreversible outcome mainly but not limited to diabetes mellitus (DM) and cardiovascular diseases (CVD). Recent publications had suggested that Leptin/Adiponectin (L/A) ratio in addition to the leptin and adiponectin as biomarkers for MetS. In the present study, the association of leptin, adiponectin and L/A ratio with MetS is determined in the middle to old aged group of local population. The objective of this project was to determine the plasma level of leptin, adiponectin and L/A ratio in a group of community subjects with and without Metabolic Syndrome (MetS) defined by the NCEP ATP III criteria with Asian specific waist circumference. Determination of the two adipokines at the age group of 30-50 was also unique in this study. Potential application of the study was the use of the two plasma adipokines as well as the L/A ratio as biomarker and predictor of MetS in the future. In the present retrospective study, one hundred thirty seven of the samples were randomly archived from the sample pool from a community-wide screening champagne for MetS initiated by the Hong Kong Polytechnic University. Commercial ELISA kits (Invitrogen Corporation, 542 Flynn road, Camarillo, CA93012, Catalog #KHP0041 and KAC2281) were used for the determination of plasma adiponectin and leptin respectively. For female group, significantly higher plasma leptin level (17.6 ng/mL versus 8.6 ng/mL, p<0.0001); lower plasma adiponectin level (6.0 μg/mL versus 10.7μg/mL, p<0.0001); and higher L/A ratio (3.59 versus 0.93, p<0.0001) were found between the MetS group and the non MetS group. For the male group, all results were found statistically not significant: with similar plasma leptin level (6.7 ng/mL versus 5.2 ng/mL, p=1.00); similar plasma adiponectin level (5.7 μg/mL versus 8.1 μg/mL, p=0.36); and similar L/A ratio (1.24 versus 0.64, p=1.00) between the MetS group and the non MetS group.
In general, significant higher plasma leptin level (14.3 ng/mL versus 7.9 ng/mL, p=0.001); lower plasma adiponectin level (5.9 μg/mL versus 10.2 μg/mL, p<0.0001); and higher L/A ratio (2.89 versus 0.87, p<0.0001) were found between the MetS group and the non MetS group. Plasma leptin was found to be positive correlated to waist circumference (r=0.435, p<0.0005), glucose (r=0.265, p=0.002) and triglyerides (r=0.351, p<0.0005); while negative correlated to HDL cholesterol (r=-0.248, p=0.003) and age (r=-0.177, p=0.038). Plasma adiponectin was found to be positive correlated to HDL cholesterol (r=0.703, p<0.0005); while negative correlated to waist circumference (r=-0.479, p<0.0005), systolic blood pressure(r=-0.387, p<0.0005), diastolic blood pressure(r=-0.335, p<0.0005), glucose (r=-0.549, p<0.0005) and triglyerides (r=-0.512, p<0.0005). The highest correlation coefficient was found in plasma adiponectin with HDL cholesterol, glucose and triglycerides. L/A ratio was found to be positively correlated to waist circumference (r=0.544, p<0.0005), systolic blood pressure(r=0.235, p=0.006), diastolic blood pressure(r=0.300, p<0.0005), glucose (r=0.477, p<0.0005) and triglyerides (r=0.539, p<0.0005); while negatively correlated to HDL cholesterol (r=-0.531, p<0.0005) and age (r=-0.177, p<0.0005). In conclusion, significant association of plasma leptin, adiponectin and L/A ratio with metabolic syndrome has been demonstrated in the present study. As there is gender difference in plasma adipokines level, gender matched comparison was made. However, only female subjects showed the significant difference in plasma leptin, adiponectin, and L/A ratio between the MetS group with the non MetS group. No significant difference was found in the two adipokines for the male subjects between the MetS group and the Non-MetS group. Therefore, plasma leptin, adiponectin and L/A ratio can be used in conjunction with other biochemical parameters for MetS diagnosis. For future study, male group subjects with larger sample size may needed. Furthermore, recruitment of younger age group of subjects and determination of high molecular weight (HMW) adiponectin may also have potential clinical value.

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