Plasma miRNA 135b expression in patients with colorectal cancer

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Plasma miRNA 135b expression in patients with colorectal cancer

 

Author: Kan, Chau Ming
Title: Plasma miRNA 135b expression in patients with colorectal cancer
Degree: M.Sc.
Year: 2016
Subject: Colon (Anatomy) -- Cancer -- Genetic aspects.
Rectum -- Cancer -- Genetic aspects.
Small interfering RNA.
Hong Kong Polytechnic University -- Dissertations
Department: Dept. of Health Technology and Informatics
Pages: xi, 70 pages : illustrations (some color)
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b2925464
URI: http://theses.lib.polyu.edu.hk/handle/200/8716
Abstract: Colorectal cancer remains the second common cancer type in worldwide. Non-invasive, cost effective, high sensitivity and specificity biomarkers can facilitate colorectal cancer detection, staging and making decision on the treatment, which can improve the mortality rate of colorectal cancer. The small non-coding ribonucleic acid molecule, microRNAs act as an important role on regulators of different diseases, including cancer. The aim of this study is to identify that miR-135b is the potential biomarkers for the early detection of the colorectal cancer by measuring the concentration of miR-135b in human plasma in various TumorNode-Metastasis stages of colorectal cancer, normal subjects and the pre & post-operative samples. By using quantitative reverse transcription polymerase chain reaction, we found that circulating miR-135b was obviously associated with the stages of colorectal cancer in 54 plasma samples. We observed that the expression levels of miR-135b were elevated from adenomas to carcinoma of colorectal cancer and higher than that in normal subjects, which demonstrated that it is potential biomarker for the early detection of colorectal cancer. Furthermore, our analysis also showed that reduction of miR-135b concentration was found in the post-operative plasma samples comparing with the pre-operative samples. We proposed that plasma miR-135b is more stable on measuring the expression levels because of the prevention of the ribonucleic acid degradation by ribonuclease enzyme. However, since the sample size in this study is small, further researches need to be done, in order to examine the sensitivity and specificity of using miR-135b as biomarkers of colorectal cancer.

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