Comparison of real-time quantitative PCR and droplet digital PCR for HIV viral load quantification

Pao Yue-kong Library Electronic Theses Database

Comparison of real-time quantitative PCR and droplet digital PCR for HIV viral load quantification

 

Author: Tse, Wing Lam Wynne
Title: Comparison of real-time quantitative PCR and droplet digital PCR for HIV viral load quantification
Degree: M.Sc.
Year: 2016
Subject: AIDS (Disease) -- Laboratory manuals.
HIV (Viruses) -- Laboratory manuals.
Hong Kong Polytechnic University -- Dissertations
Department: Dept. of Health Technology and Informatics
Pages: vii, 40 pages : color illustrations
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b2925467
URI: http://theses.lib.polyu.edu.hk/handle/200/8719
Abstract: Assessment of HIV viral load is one of the best predictors of clinical disease progression and it is the main parameter used to evaluate treatment success in HIV-positive patients. Maintenance of an undetectable viral load is fundamental so as to know we are moving in the right direction towards long-term HIV suppression and even eradication in the battle with HIV. Otherwise, inappropriate clinical management as well as the selection and accumulation of residual drug-resistant viruses and subsequent transmission within the population could result in inevitable consequences. In the present study, the performance of real-time quantitative PCR (qPCR) and droplet digital PCR (ddPCR) for HIV viral load quantification was evaluated. Plasmid DNA extracted from pNL4-3 and RNA from the Qnostics HIV-1 Evaluation Panel was used for evaluation. The result of the novel platform, ddPCR, found no better than qPCR, the most frequently used method currently. The proposed improvement in accuracy, sensitivity and precision for low input target concentration over qPCR would await further approval.

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