Full metadata record
DC FieldValueLanguage
dc.contributorDepartment of Health Technology and Informaticsen_US
dc.contributor.advisorLeung, Polly (HTI)-
dc.creatorTse, Wing Lam Wynne-
dc.identifier.urihttps://theses.lib.polyu.edu.hk/handle/200/8719-
dc.languageEnglishen_US
dc.publisherHong Kong Polytechnic University-
dc.rightsAll rights reserveden_US
dc.titleComparison of real-time quantitative PCR and droplet digital PCR for HIV viral load quantificationen_US
dcterms.abstractAssessment of HIV viral load is one of the best predictors of clinical disease progression and it is the main parameter used to evaluate treatment success in HIV-positive patients. Maintenance of an undetectable viral load is fundamental so as to know we are moving in the right direction towards long-term HIV suppression and even eradication in the battle with HIV. Otherwise, inappropriate clinical management as well as the selection and accumulation of residual drug-resistant viruses and subsequent transmission within the population could result in inevitable consequences. In the present study, the performance of real-time quantitative PCR (qPCR) and droplet digital PCR (ddPCR) for HIV viral load quantification was evaluated. Plasmid DNA extracted from pNL4-3 and RNA from the Qnostics HIV-1 Evaluation Panel was used for evaluation. The result of the novel platform, ddPCR, found no better than qPCR, the most frequently used method currently. The proposed improvement in accuracy, sensitivity and precision for low input target concentration over qPCR would await further approval.en_US
dcterms.extentvii, 40 pages : color illustrationsen_US
dcterms.isPartOfPolyU Electronic Thesesen_US
dcterms.issued2016en_US
dcterms.educationalLevelAll Masteren_US
dcterms.educationalLevelM.Sc.en_US
dcterms.LCSHAIDS (Disease) -- Laboratory manuals.en_US
dcterms.LCSHHIV (Viruses) -- Laboratory manuals.en_US
dcterms.LCSHHong Kong Polytechnic University -- Dissertationsen_US
dcterms.accessRightsrestricted accessen_US

Files in This Item:
File Description SizeFormat 
b2925467x.pdfFor All Users (off-campus access for PolyU Staff & Students only)1.63 MBAdobe PDFView/Open


Copyright Undertaking

As a bona fide Library user, I declare that:

  1. I will abide by the rules and legal ordinances governing copyright regarding the use of the Database.
  2. I will use the Database for the purpose of my research or private study only and not for circulation or further reproduction or any other purpose.
  3. I agree to indemnify and hold the University harmless from and against any loss, damage, cost, liability or expenses arising from copyright infringement or unauthorized usage.

By downloading any item(s) listed above, you acknowledge that you have read and understood the copyright undertaking as stated above, and agree to be bound by all of its terms.

Show simple item record

Please use this identifier to cite or link to this item: https://theses.lib.polyu.edu.hk/handle/200/8719