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dc.contributorDepartment of Applied Biology and Chemical Technologyen_US
dc.creatorCheng, Chun-kwok-
dc.identifier.urihttps://theses.lib.polyu.edu.hk/handle/200/936-
dc.languageEnglishen_US
dc.publisherHong Kong Polytechnic University-
dc.rightsAll rights reserveden_US
dc.titleA study of endogenous proteinaceous regulators of plasma membrane calcium-ATPase in porcine erythrocyteen_US
dcterms.abstractPlasma membrane calcium ATPase (PMCA) is an important enzyme involved in many vital regulatory processes. Alternation in erythrocyte PMCA has been observed in several diseases conditions. The purpose of this study is to evaluate the interaction of endogenous proteinaceous regulators, namely the activator calmodulin (CaM) and plasma membrane calcium-ATPase inhibitor (PMCAI), with intact porcine erythrocytes; and to see if they can be potential candidates for therapeutic use in diseases with calcium ion transport problems. Biochemical and morphological changes of intact young and old porcine erythrocytes, and endogenous proteinaceous regulators-fused porcine erythrocytes upon calcium and glucose challenges were studied in this regard. In our study, sodium-potassium-ATPase (Na+/K+-ATPase) of young porcine erythrocyte was found to be more effective than old porcine erythrocyte in pumping out sodium ion and potassium into the cell. The function of Na+/K+-ATPase is to maintain a high potassium concentration and a low sodium concentration in cytosol when cell is challenged with high extracellular calcium. Difference in calcium- ATPase activities between young and old porcine erythrocyte populations, however, was not observed. When porcine erythrocyte was fused with phosphate-buffered saline, CaM, or PMCAI, Na+/K+-ATPase of porcine erythrocyte was found still active in pumping sodium ion out and potassium into the cell. However, noticeable hemoglobin leakage and cell lysis were observed in PMCAI-fused porcine erythrocyte incubated in high calcium level at 37C for four hours indicating that PMCAI was active in inhibiting the calcium-ATPase and subsequently affected the membrane integrity. Further works have to be done to study and differentiate the effect that is purely caused by endogenous proteinaceous regulators in the cytosol, and/or in the plasma, or possibly from the lyzed membrane protein.en_US
dcterms.extentvii, 114 leaves : ill. (some col.) ; 30 cmen_US
dcterms.isPartOfPolyU Electronic Thesesen_US
dcterms.issued2003en_US
dcterms.educationalLevelAll Masteren_US
dcterms.educationalLevelM.Sc.en_US
dcterms.LCSHHong Kong Polytechnic University -- Dissertationsen_US
dcterms.LCSHCalcium -- Physiological effecten_US
dcterms.LCSHPlant cell membranesen_US
dcterms.LCSHAdenosine triphosphataseen_US
dcterms.LCSHCalcium channelsen_US
dcterms.accessRightsrestricted accessen_US

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Please use this identifier to cite or link to this item: https://theses.lib.polyu.edu.hk/handle/200/936