Polyhydroxyalkanoates production by recombinant E. coli BL1-Blue Ampr, E. coli HMS174 Ampr, A. latus DSM1123 and A. latus DSM1124 using medium chain length fatty acid as sole carbon source

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Polyhydroxyalkanoates production by recombinant E. coli BL1-Blue Ampr, E. coli HMS174 Ampr, A. latus DSM1123 and A. latus DSM1124 using medium chain length fatty acid as sole carbon source

 

Author: Wong, Ming-yan
Title: Polyhydroxyalkanoates production by recombinant E. coli BL1-Blue Ampr, E. coli HMS174 Ampr, A. latus DSM1123 and A. latus DSM1124 using medium chain length fatty acid as sole carbon source
Degree: M.Sc.
Year: 2002
Subject: Hong Kong Polytechnic University -- Dissertations
Poly-beta-hydroxyalkanoates
Department: Multi-disciplinary Studies
Dept. of Applied Biology and Chemical Technology
Pages: xiii, 72 leaves : ill. ; 30 cm
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b1615448
URI: http://theses.lib.polyu.edu.hk/handle/200/937
Abstract: To investigate the ability of recombinant E. coli BL1-Blue Ampr , E. coli HMS 174 Ampr, Alcaligenes latus DSM1123 and Alcaligenes latus DSM1124 to utilize medium chain length fatty acid, capric and oleic acid as sole carbon source for PHA production, the strains were cultivated at 37C orbital shaker in l00mL medium E (Cromwick, Foglia, Lenz, 1996) containing 0.2% w/v capric, 0.2%, 0.5% and 1.0% w/v oleic acid. All the four strains of the bacteria were found sustainable in the medium mentioned above with different grow and PHA production characteristics. Fed batch fermentation was more effective for both cell grow and PHA production. It was found that Alcaligenes latus DSM1124 produced the highest amount of PHB in 1.0%w/v oleic acid medium. The amount of PHB produced was 4.43mg from l00mL medium. Reducing the concentration of the medium would reducing the amount of PHB produced. 0.2% w/v oleic acid medium was not sufficient to support cell grow for the four strains of bacteria due to nutrient depletion. The finding was different from capri acid, PHB and PHV was produced by all the four strains of bacteria in capric acid medium with the concentration as low as 0.2% w/v. The amount of PHB produced from recombinant E.coil BLi-Blue Ampr was 0.71mg, 0.36% product yield, 0.50mg, 0.25% product yield from recombinant E. coli HMS174 Ampr, 0.53mg, 0.27% product yield from A. latus DSM1123 and 1.29mg, 0.65% product yield from A. latus DSM1124 in l00mL 0.2% w/v medium each. The result showed that capric acid was more effective for PHA production.

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