| Author: | Ng, Po Wah Lawrence |
| Title: | Analysis of MYB expression and MYB translocations in salivary gland and lacrimal gland adenoid cystic carcinomas |
| Degree: | M.Sc. |
| Year: | 2014 |
| Subject: | DNA-binding proteins. Adenoid cystic carcinoma Genetic transcription. Hong Kong Polytechnic University -- Dissertations |
| Department: | Department of Health Technology and Informatics |
| Pages: | x, 63 leaves : color illustrations ; 30 cm |
| Language: | English |
| Abstract: | A subset of Adenoid Cystic Carcinoma (ACC) is characterized by MYB fusions including the novel t(6;9)(q22-23; p23-24) translocation. There has been over expression in MYB RNA in ACCs regardless to MYB fusions. Retrospective study of formalin-fixed paraffin embedded (FFPE) samples of 21 salivary gland ACCs and 3 lacrimal gland ACCs together with 6 non-ACC salivary gland tumors were studied. As a result, MYB-NFIB fusion transcripts in salivary gland adenoid cystic carcinoma and lacrimal gland carcinoma were 9% and 50% respectively with an overall frequency of 15%. No MYB-NFIB fusion transcripts were identified in all non-ACC of salivary gland, suggestive of a high specificity of MYB-NFIB fusion in ACC of salivary gland. Sixty-seven percent of salivary gland ACC were MYB fusion positive. In case of lacrimal gland ACC, all cases were fusion positive. The overall frequency of MYB fusions was therefore 71%. MYB expression was found in 80% and 67% of fusion-positive salivary gland and fusion-positive lacrimal gland ACCs and was about 67% of fusion-negative salivary gland ACCs despite of low expression in fusion-negative ACCs. MYB over expression didn't appear in other salivary gland tumors. Overall percentage of MYB expression in ACC samples was 75% whereas the percentages of MYB expression in fusion positive and negative ACC samples were 78% and 67% respectively. Hence, MYB can be counted as a major oncogene in ACC pathogenesis. |
| Rights: | All rights reserved |
| Access: | restricted access |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| b2759015x.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 912.62 kB | Adobe PDF | View/Open |
Copyright Undertaking
As a bona fide Library user, I declare that:
- I will abide by the rules and legal ordinances governing copyright regarding the use of the Database.
- I will use the Database for the purpose of my research or private study only and not for circulation or further reproduction or any other purpose.
- I agree to indemnify and hold the University harmless from and against any loss, damage, cost, liability or expenses arising from copyright infringement or unauthorized usage.
By downloading any item(s) listed above, you acknowledge that you have read and understood the copyright undertaking as stated above, and agree to be bound by all of its terms.
Please use this identifier to cite or link to this item:
https://theses.lib.polyu.edu.hk/handle/200/7595