Molecular detection of vancomycin tolerant Streptococcus pneumoniae in Hong Kong

Pao Yue-kong Library Electronic Theses Database

Molecular detection of vancomycin tolerant Streptococcus pneumoniae in Hong Kong

 

Author: Wong, Chun-kuen
Title: Molecular detection of vancomycin tolerant Streptococcus pneumoniae in Hong Kong
Degree: M.Sc.
Year: 2010
Subject: Hong Kong Polytechnic University -- Dissertations
Vancomycin resistance -- China -- Hong Kong
Streptococcus pneumoniae -- China -- Hong Kong
Department: Faculty of Health and Social Sciences
Pages: 54 leaves : ill. ; 30 cm.
InnoPac Record: http://library.polyu.edu.hk/record=b2356831
URI: http://theses.lib.polyu.edu.hk/handle/200/5700
Abstract: New rapid methods for highly sensitive and specific detection of vancomycin tolerant pneumococci may be able to improve the diagnosis and management of antimicrobial pneumococcal infection but are also important for antibiotic treatment studies and vaccine development. The purpose of this study was to develop a sensitive and specific PCR method for the detection of vancomycin tolerance in S. pneumoniae and to study the applicability of these methods to detecting vancomycin tolerant type pneumococci from clinical samples. A PCR assay was established for detecting pneumococcal pep 27 DNA, and this pep27 is believed to be absent or in mutated form in most vancomycin tolerant pneumococcal strains. By determination of gene pep27 with specific primers, we were able to rapidly identify vancomycin tolerance in clinical samples and this method could replace the cumbersome cell culture method. The sensitivity and specificity of PCR assay were found to be 100% and 71.4% respectively on the basis of the results of time kill curve method. We conclude that pep 27 PCR may be useful for rapid detection of vancomycin tolerant Streptococcus pneumoniae which could allow for appropriate treatment of seriously ill patients to be commenced promptly.

Files in this item

Files Size Format
b23568318.pdf 3.290Mb PDF
Copyright Undertaking
As a bona fide Library user, I declare that:
  1. I will abide by the rules and legal ordinances governing copyright regarding the use of the Database.
  2. I will use the Database for the purpose of my research or private study only and not for circulation or further reproduction or any other purpose.
  3. I agree to indemnify and hold the University harmless from and against any loss, damage, cost, liability or expenses arising from copyright infringement or unauthorized usage.
By downloading any item(s) listed above, you acknowledge that you have read and understood the copyright undertaking as stated above, and agree to be bound by all of its terms.

     

Quick Search

Browse

More Information