Polyethylene-glycol hydrogel scaffold conjugated with cell adhesion peptide for cell culture

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Polyethylene-glycol hydrogel scaffold conjugated with cell adhesion peptide for cell culture


Author: Chan, Chun Yu Ron
Title: Polyethylene-glycol hydrogel scaffold conjugated with cell adhesion peptide for cell culture
Degree: M.Sc.
Year: 2012
Subject: Polyethylene glycol.
Cell adhesion.
Hong Kong Polytechnic University -- Dissertations
Department: Dept. of Health Technology and Informatics
Pages: xiv, 50 leaves : ill. (some col.) ; 30 cm.
Language: English
InnoPac Record: http://library.polyu.edu.hk/record=b2520213
URI: http://theses.lib.polyu.edu.hk/handle/200/6557
Abstract: Results of nerve damage various; including loss of sensation, motor movement impairment, which depends on the site of incision. Traditionally, nerve severance was treated by autograph, requiring the surgeon to extract another segment of nerve from the patient. This will create another opening wound on the patient and the healing of nerve may not be satisfying. Tissue engineering is a very hot research topic providing new cues in treating nerve severance. Nerve guidance conduit, which is basically a cell culture scaffold, could help to guide and stimulate nerve regeneration. Poly (ethylene-glycol) hydrogel, which is highly hydrophilic, biocompatible, non-toxic and wildly applied in medical treatment, was considered as a potential material for fabricating nerve guidance conduit. It is often described as a "blank slate", while properties can be "written" on it. The tunable characteristic made it very suitable for being a nerve guidance conduit. In this study, cell adhesion peptide, a component in extracellular matrix, was immobilized in poly (ethylene-glycol). The cell adhesion peptide provided binding site for integrin receptors on cell surface, allowing cells to bind on a hydrophilic hydrogel. Experimental result demonstrated that when the cell adhesion peptide concentration in hydrogel reached 5.3μmol/cm³, neurite extension activity was found on cells seeded on it. This exploratory study verified the capability of cell adhesion peptide modified four-arm poly (ethylene-glycol) hydrogel of being a cell culturing scaffold which can support neurite extension. In future, it could become a wildly used tissue engineering scaffold thanks for its biocompatibility.

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