| Author: | Yong, Fung-mei |
| Title: | Proteome approach in identification of novel tumour markers in patients with cancer |
| Degree: | M.Sc. |
| Year: | 2001 |
| Subject: | Tumor markers Hong Kong Polytechnic University -- Dissertations |
| Department: | Multi-disciplinary Studies Department of Applied Biology and Chemical Technology |
| Pages: | ix, 83 leaves : ill. (some col.) ; 30 cm |
| Language: | English |
| Abstract: | Current common diagnostic methods for tumour are either invasive in nature or involving exposure to radiation. Compared with healthy individuals, tumour patients have elevated level of some proteins in serum due to uncontrolled proliferation. Presence of these proteins can be used as tumour markers. This work involves studies of the changes in two-dimensional polyacrylamde gel electrophoresis (2D-PAGE) patterns of the sera samples from patients with lung carcinoma, stomach carcinoma and colon carcinoma. The aim is to identify new tumour markers in order to facilitate early detection and diagnosis. Tumour marker is defined as the protein spot only present in the 2D-PAGE gels of sera from tumour patients but not from healthy individuals. It is found that there are five probable tumour markers (pI/Mr,=4.8/60kDa, 5.5/60kDa, 5.6/60kDa, 5.6/52kDa, 5.7/52kDa) for stomach adenocarcinoma. Nine probable markers are found (4.8/60kDa, 5.4/60kDa, 5.5/60kDa, 5.6/60kDa, 5.5/52kDa 5.6/52kDa 5.7/52kDa 6.4/45kDa, 6.5/45kDa) for stomach adenosquamous carcinoma; two others (5.7/60kDa, 5.9/52kDa) for colon adenocarcinoma. In addition, three markers (4.8/60kDa, 5.2/60kDa 5.7/52kDa) for squamous cell lung carcinoma and six (4.8/60kDa, 6.5/56kDa, 6.6/54kDa, 6.8/55kDa, 5.7/52kDa 6.0/28kDa) for large cell lung anaplastic carcinoma. Among these, it can be found that all the sera samples with carcinoma appear to have a same spot with a pI/Mr of 5.7/52kDa. Therefore the spot may be a general marker for occurrence of tumour. Moreover, other protein spots with pI 5.8-6.0, Mr 45kDa are commonly present in normal serum but absent in 7 out of 9 tumour images. Furthermore the spot at 4.8/60kDa is present in two cases in stomach carcinoma and two cases in lung carcinoma but not the others and yet another spot at 5.4/55kDa is absent in some cases randomly. Further investigation to identify these markers is necessary. |
| Rights: | All rights reserved |
| Access: | restricted access |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| b15533517.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 5.21 MB | Adobe PDF | View/Open |
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