Author: Piao, Yun
Title: GelMA hydrogels as tissue engineering scaffolds
Advisors: Zhao, Xin (BME)
Degree: M.Phil.
Year: 2022
Subject: Tissue scaffolds
Tissue engineering
Hong Kong Polytechnic University -- Dissertations
Department: Department of Biomedical Engineering
Pages: xx, 82 pages : color illustrations
Language: English
Abstract: Gelatin methacryloyl (GelMA) hydrogels could be obtained by a concise crosslinking process induced by light with specific wavelengths, and possess superior biocompatibility due to various functional motifs with cell-adhesive and MMP-degradable characteristics, and controllable mechanical and biological properties by adjusting the concentration of crosslinked unsaturated bonds. Electrohydrodynamic atomization, including electrospraying, electronetting, and electrospinning, can produce scaffolds with various three-dimensional (3D) structures, physical properties, and chemical properties. In the present study, using electrospinning technique, a novel hairy HA (methacrylate modified hydroxyapatite, HAMA) reinforced GelMA electrospun fibrous membranes (EFMs) will be proposed as an artificial periosteum and be realized via a co-crosslinked double network, combining both inorganic and organic components as periosteum for bone regeneration. We found that the novel artificial periosteum promotes both osteogenesis and angiogenesis, and the effect is significantly stronger than the separated organic or inorganic part. This shows the novel artificial periosteum with an organic-inorganic co-crosslinked network is not a simple combination of the advantages of the single factors but a generation of synergy effect. We have also used the electrospraying technique, assisted by microfluidic devices, to create porous microcarriers on a large scale encapsulating NK cells for tumor immunotherapy. We found that the NK-92MI cells encapsulated microcarriers can destroy tumor cells at an early stage by secreting these factors, and with the proliferation and migration, the cells could directly affect tumor tissue at the long-term stage. The microcarriers could also work as a shield to protect incorporated NK cells from the immune response while the release of the tumor-killing factors is not affected. The attempts of artificial periosteum and NK cells incorporated microcarriers investigate the biological application of GelMA hydrogel assisted by electrohydrodynamic atomization technique.
Rights: All rights reserved
Access: open access

Files in This Item:
File Description SizeFormat 
6142.pdfFor All Users5.51 MBAdobe PDFView/Open

Copyright Undertaking

As a bona fide Library user, I declare that:

  1. I will abide by the rules and legal ordinances governing copyright regarding the use of the Database.
  2. I will use the Database for the purpose of my research or private study only and not for circulation or further reproduction or any other purpose.
  3. I agree to indemnify and hold the University harmless from and against any loss, damage, cost, liability or expenses arising from copyright infringement or unauthorized usage.

By downloading any item(s) listed above, you acknowledge that you have read and understood the copyright undertaking as stated above, and agree to be bound by all of its terms.

Show full item record

Please use this identifier to cite or link to this item: