| Author: | Pong, Wing Ka |
| Title: | Evaluation of the effect of S100 Calcium Binding Protein A4 (S100A4) on blood triglyceride and glucose level in obese mice |
| Advisors: | Wong, Chi-ming (HTI) |
| Degree: | M.Sc. |
| Year: | 2022 |
| Subject: | Calcium-binding proteins Obesity Hong Kong Polytechnic University -- Dissertations |
| Department: | Department of Health Technology and Informatics |
| Pages: | 48 pages : color illustrations |
| Language: | English |
| Abstract: | Introduction: Obesity is a worldwide health concern and its prevalence is rising not only in adults but also in children. Impaired adipogenesis and adipocyte dysfunction in obesity may result in comorbidities such as insulin resistance, type II diabetes, dyslipidaemia and cardiovascular disease. Uncontrolled obesity is life-threatening and thus management of obesity is crucial. However, there is a limited number of pharmacological therapy available nowadays, exploring new therapeutic targets is therefore in need. S100 Calcium Binding Protein A4 (S100A4) protein is a member of the S100 protein family and is a calcium-binding protein. The protein is believed to somehow relate with obesity as protective effects has been observed and expression of S100A4 changed in obese subjects. In vivo studies to investigate the effect of S100A4 on blood triglyceride and glucose were performed to hopefully provide an insight into the association between S100A4 and obesity and explore the possibility of S100A4 being a therapeutic target. Aims: The purpose of this study is to extract and purify S100A4 protein and to evaluate its effects on blood triglyceride and glucose level in obese mice. Method: Extraction of histidine-tagged S100A4 protein was performed and purified by the nickel-nitrilotriacetic (Ni-NTA) column. Mice with a high fat diet were fasted and divided into two groups: S100A4 and control. Intraperitoneal injection of S100A4 and control was performed. Triglyceride level at 0-, 0.5-, 1-, 2-, 3- and 24-hour was measured. Glucose challenge test was performed and measurements were done every 15 minutes for an hour and at 90-minute. Data collected in both studies was then analysed using statistical methods to look for significance difference. Results: S100A4 protein was successfully extracted and purified by Ni-NTA column and 5 mg/mL protein was obtained. There is no significant difference found in triglyceride level between the S100A4 and the control group while significant decrease of glucose level by S100A4 can be observed. Conclusion: The extraction and purification of S100A4 is successful. Data obtained in triglyceride study is inconclusive and there is no observable correlation found between S100A4 and triglyceride level. Several improvements have been suggested for the triglyceride experiment such as increasing sample size and dosage. S100A4 is found to lower glucose level in vivo and thus can be a potential therapeutic target for management of obesity. |
| Rights: | All rights reserved |
| Access: | restricted access |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 6528.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 2.19 MB | Adobe PDF | View/Open |
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