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dc.contributorDepartment of Biomedical Engineeringen_US
dc.contributor.advisorTan, Youhua (BME)en_US
dc.creatorLiu, Yutian-
dc.identifier.urihttps://theses.lib.polyu.edu.hk/handle/200/12936-
dc.languageEnglishen_US
dc.publisherHong Kong Polytechnic Universityen_US
dc.rightsAll rights reserveden_US
dc.titleInvestigating the effect of Nivolumab drug on the adhesion of Jurkat cells and SK-MEL-28 cells and the degree of T cell activationen_US
dcterms.abstractThe adhesion of Jurkat cells to SK-MEL-28 was assessed by using fluorescence staining at different Nivolumab concentrations, and the difference in the adhesion of Jurkat cells to SK-­MEL-28 under different conditions was indirectly measured by the relationship between flow rate and shear force. The changes in the degree of Jurkat cell activation during this process were also assessed using flow cytometry and other means, in order to explore the possible relationship between the adhesion force and the degree of T cell activation. Based on our experiments, it was found that the expression of PD-L1 on SK-MEL-28 cells may reduce the adhesion force of Jurkat cells. But, the adhesion capacity of Programmed Cell Death Protein 1 (PD-1)-expressing Jurkat cells with Programmed Death Ligand 1 (PD-L1)-expressing SK­-MEL-28 cells may be restored through the effect of Nivolumab, which may be somehow related to the restoration of the T cell activation capacity. correlation. Therefore, it may also indicate that Nivolumab has therapeutic and applied value for the treatment of skin metastatic melanoma.en_US
dcterms.extent36 pages : color illustrationsen_US
dcterms.isPartOfPolyU Electronic Thesesen_US
dcterms.issued2024en_US
dcterms.educationalLevelM.Sc.en_US
dcterms.educationalLevelAll Masteren_US
dcterms.LCSHImmunotherapyen_US
dcterms.LCSHT cellsen_US
dcterms.LCSHCell adhesion moleculesen_US
dcterms.LCSHMelanoma -- Treatmenten_US
dcterms.LCSHHong Kong Polytechnic University -- Dissertationsen_US
dcterms.accessRightsrestricted accessen_US

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Please use this identifier to cite or link to this item: https://theses.lib.polyu.edu.hk/handle/200/12936