| Author: | Xu, Jiahao |
| Title: | Characterization of mechanical properties during cell to cancer cell transformation |
| Advisors: | Tan, Youhua (BME) |
| Degree: | M.Sc. |
| Year: | 2024 |
| Subject: | Cell transformation Cancer cells Cells -- Mechanical properties Hong Kong Polytechnic University -- Dissertations |
| Department: | Department of Biomedical Engineering |
| Pages: | 37 pages : color illustrations |
| Language: | English |
| Abstract: | Mechanical changes during cell transformation, especially changes in cytoskeleton hardness, cell traction, and actin-filament composition, are an area that has not been fully studied. These changes in mechanical properties not only affect the behavior and function of cells but also may be closely related to the carcinogenesis of cells. Many studies have focused on how these changes in mechanical properties affect cell transformation, but no one has explored the detailed relationship between mechanical properties over time during the transformation of cells into cancer cells. The purpose of this study is to characterize the relationship between mechanical properties and time in the process of cell transformation, which is mainly divided into two stages. The first stage is the study of the process of cell transformation, and the progress of cell transformation is judged by using the expression of phosphorylated ERK protein that cancer cells can mitosis in a serum-free environment while ordinary cells cannot. The second stage is to find specific time points after exploring the detailed transformation of cells over time and explore the changes in mechanical properties at these time points, mainly including the detection of cytoskeleton stiffness, F-actin content, and cell traction force. My research shows that the cell transformation process goes through a process in which the stiffness first increases and then decreases while the traction gradually increases. On this basis, it is possible to explore in the future whether the carcinogenesis process of cells can be prevented or delayed by intervening with changes in the mechanical properties of cells at these specific time points. A deeper understanding of this area of research not only has important implications for basic cell biology but may also provide new strategies and approaches for the diagnosis and treatment of cancer. I hope this paper will provide a new perspective for the study of the mechanical properties of cells and provide new possibilities for preventing cell carcinogenesis. |
| Rights: | All rights reserved |
| Access: | restricted access |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| 7423.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 856.96 kB | Adobe PDF | View/Open |
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