Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor | Faculty of Health and Social Sciences | en_US |
| dc.creator | Jin, Cheng | - |
| dc.identifier.uri | https://theses.lib.polyu.edu.hk/handle/200/13546 | - |
| dc.language | English | en_US |
| dc.publisher | Hong Kong Polytechnic University | en_US |
| dc.rights | All rights reserved | en_US |
| dc.title | Pathological changes of the dorsal root ganglion in experimental autoimmune myositis | en_US |
| dcterms.abstract | Introduction: Idiopathic inflammatory myositis (IIM) is an autoimmune disease with unclear pathological mechanisms. The impacts of IIM extend beyond the physical symptoms to include various problems, such as financial instability, social isolation, and mental health, and this highlights the importance of studying the disease for both medical and societal reasons. The primary symptoms of IIM patients include decreased muscle strength and pain. Currently, research on IIM has mainly focused on the immune system only, although a few reports have investigated the nervous sensory system. The primary sensory neuron body is located in the dorsal root ganglion (DRG). The DRG is responsible for sensation perception and is involved in neuroimmune regulation. According to reports from other diseases, fatigue, and sensation change may be related to pathological changes in the DRG. This study aimed to reveal the pathological changes in the DRG in the experimental autoimmune myositis (EAM) mice model. | en_US |
| dcterms.abstract | Method: We used eight-week-old male C57/BL 6J mice to develop the EAM model through subcutaneous injection of myosin and fredrin complete adjuvant, the EAM sham group through subcutaneous injection of phosphate buffer salt solution, and the wild-type (WT) group, with ten mice in each group. The body weight, grip strength, and mechanism threshold were measured at baseline and four weeks after initial modeling. Hematoxylin and Eosin (H&E) staining and immunofluorescence were used to observe the morphology of the DRG in all groups. The between-group differences in various outcome variables were analyzed using a separate one-way ANOVA with Turkey's post hoc test. | en_US |
| dcterms.abstract | Results: The EAM mice demonstrated a significant decrease in average grip strength (EAM, 0.99 ± 0.16 N; EAM sham, 1.29 ± 0.14 N; WT, 1.43 ± 0.08 N) and mechanical thresholds (EAM, 0.21 ± 0.08; EAM sham, 0.80 ± 0.12; WT, 1.15 ± 0.13) compared to the EAM sham and control group respectively (n = 10, P < 0.05). The muscle and DRG tissue structure were disorganized in the EAM group, and the number of platelet endothelial cell adhesion molecule 1 (CD31) + cell and calcitonin gene-related peptide (CGRP) + neurons were significantly increased in the DRG of the EAM group compared to the other two groups. | en_US |
| dcterms.abstract | Conclusion: Administering 1 mg myosin four times subcutaneously in the mice resulted in muscle inflammation and decreased grip and mechanical thresholds in EAM mice. The up-regulated CGRP and CD31 among the DRG may partially demonstrate the sensory nerve abnormalities in EAM. We hope these findings provide a potential theoretical framework for further investigation in patients with IIM. | en_US |
| dcterms.extent | iii, 204 pages : color illustrations | en_US |
| dcterms.isPartOf | PolyU Electronic Theses | en_US |
| dcterms.issued | 2024 | en_US |
| dcterms.educationalLevel | DHSc | en_US |
| dcterms.educationalLevel | All Doctorate | en_US |
| dcterms.accessRights | restricted access | en_US |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 7996.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 2.19 MB | Adobe PDF | View/Open |
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