Full metadata record
|dc.contributor||Department of Applied Biology and Chemical Technology||en_US|
|dc.publisher||Hong Kong Polytechnic University||-|
|dc.rights||All rights reserved||en_US|
|dc.title||Structure and function relationship of porcine pyridoxal kinase||en_US|
|dcterms.abstract||Recombinant truncated mutants of pyridoxal kinase lacking a series of amino acid residues at the N-terminal domain have been constructed. Deletion of 15 amino acid residues does not inactivate the kinase, whereas deletion of 16, 17, 18, and 21 or more amino acid residues, pertaining to the highly conserved sequence, RVLSIQHV, abolishes the catalytic function of the expressed enzyme. All species of recombinant pyridoxal kinase were purified using a method that a chain of histidine residues were tagged at the N-terminal of the protein. Wildtype, Δ15 and Δ16 variants of pyridoxal kinase were puritied to homogeneity by two chromatographic steps including metal-chelating and DEAE ion exchange chromatographies. Purified enzymes were characterized by using circular dichroism (CD) and fluorescence spectroscopy with respect to the folding pattern of the protein. Results indicate that deletion of 16 amino acid residues initiates a misfolding pattern for the protein leading to subsequent loss of their ability to bind an ATP analogue, Trinitrophenyl-ATP (TNP-ATP), in vitro. Thc stability of wild-type and Δ15 mutant in the presence of guandinium hydrochloride (GdnHCl) was also examined using fluorescence spectroscopy. Results have shown that the unfolding process of the wild-type protein proceeds through a concerted reaction, whereas the denaturation process of the mutants (Δ15 and Δ16) endowed with and without catalytic activity shows a biphasic pattern of unfolding in GdnHCI. It is concluded that deletion mutants of pyridoxal kinase are not only less stable than the wild-type species, but they also follow different folding pathways.||en_US|
|dcterms.extent||, 152 leaves : ill. ; 30 cm||en_US|
|dcterms.isPartOf||PolyU Electronic Theses||en_US|
|dcterms.LCSH||Enzymes -- Analysis||en_US|
|dcterms.LCSH||Proteins -- Analysis||en_US|
|dcterms.LCSH||Hong Kong Polytechnic University -- Dissertations||en_US|
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