| Author: | Chan, Yin-leung |
| Title: | Establishment of an in-vitro artificial crystallizer to determine the effects of urinary macromolecules on the risks of renal stone diseases |
| Degree: | M.Sc. |
| Year: | 2001 |
| Subject: | Urine -- Analysis Calcium oxalate Hong Kong Polytechnic University -- Dissertations |
| Department: | Multi-disciplinary Studies Department of Nursing and Health Sciences |
| Pages: | xi, 77 leaves : ill. ; 30 cm |
| Language: | English |
| Abstract: | Urinary macromolecules, originated from connective tissue, are thought to be involved in controlling crystal formation in the cases of urolithiasis. This work was aimed at studying the major family of this macromolecules found in urine, glycosaminoglycans(GAGs), that may control crystal formation from calcium and oxalate ions in the environment similar to renal milieu. To conduct the investigation of the effect of the glycosaminoglycans on the crystallization of calcium oxalate, a Mixed Suspension Mixed Product Removal (MSMPR) crystalliser was established. Two identical MSMPR chambers were set up to contain 20 mL of artificial urine with the other chamber serving as a control. By mean of this system, nucleation rate and growth rate of calcium oxalate crystals were determined by using the Coulter Multisizer counter. By introducing various concentrations of commercial GAGs, heparan sulphate(HS), dermatan sulphate(DS), chondroitin sulphate(CS) and hyaluronic acid(HA) into the test chamber, the effects of these components on crystallization of calcium oxalate in terms of nucleation and growth rate were then be evaluated. It was found that heparan sulphate enhanced nucleation rate and suppressed the growth rate on crystallization. Dermatan sulphate inhibited the growth of crystal but enhanced nucleation at low concentration. The chondrontin sulphate and hyaluronic acid gave contradictory results between low and high concentration. Chondrontin sulphate promoted growth at low concentration but not in high concentration. The reverse was the picture of hyaluronic acid, and at high concentration of hyaluronic acid, it suppressed the nucleation and promote the growth of calcium oxalate crystallization. |
| Rights: | All rights reserved |
| Access: | restricted access |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| b15732538.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 2.67 MB | Adobe PDF | View/Open |
Copyright Undertaking
As a bona fide Library user, I declare that:
- I will abide by the rules and legal ordinances governing copyright regarding the use of the Database.
- I will use the Database for the purpose of my research or private study only and not for circulation or further reproduction or any other purpose.
- I agree to indemnify and hold the University harmless from and against any loss, damage, cost, liability or expenses arising from copyright infringement or unauthorized usage.
By downloading any item(s) listed above, you acknowledge that you have read and understood the copyright undertaking as stated above, and agree to be bound by all of its terms.
Please use this identifier to cite or link to this item:
https://theses.lib.polyu.edu.hk/handle/200/2960