|Author:||Chan, Wing-chuen Gary|
|Title:||Histone H2B genes of the mole rats|
Hong Kong Polytechnic University -- Dissertations
Department of Applied Biology and Chemical Technology
|Pages:||49,  leaves : ill. ; 30 cm|
|Abstract:||Histone H2B genes of blind subterranean mole rat, the Spalax ehernbergi superspecies in Israel were characterized from twelve populations. Twelve populations of the histone H2B genes were amplified, nine populations of the genes were cloned and characterized and seven populations of the genes were sequenced. Histone H2B gene is found to be 381 bp in length and 127 amino acids in translated sequences. It is conserved within the chromosomal species with high percentage of identity (88%-98%) while highest percentage of identity is within 2n= 60 species. The highest percentage of nucleotide usage is C (30%-32%), followed by G, A and T. The highest percentage of two base combination is G + C (60%-6l%). The usage of nucleotides is relatively unbiased at first and second positions but biased towards C and G at third position. The H2B gene is lysine rich but no tryptophan is utilized in amino acid usage. The codon usages are nonrandom and mostly biased towards G in the third position, followed by C, T and A. More synonymous changes than nonsynonymous changes are found within the species that are related to the positional difference of nucleotides at third position of codons. There is a higher homogeneity within population, 2n = 60 and a higher heterogeneity within populations 2n = 52 and 2n = 58 species. Finally, within each chromosomal species. the lowest percentage of amino acid divergence is population 2n = 60, followed by 2n = 52 and 2n = 58. Several characteristics of the H2B DNA sequences in the Spalax ehrenbergi superspecies were compared with that of mouse and human as well.|
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