Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor | Department of Health Technology and Informatics | en_US |
| dc.creator | Chu, Chau-hung | - |
| dc.identifier.uri | https://theses.lib.polyu.edu.hk/handle/200/5842 | - |
| dc.language | English | en_US |
| dc.publisher | Hong Kong Polytechnic University | - |
| dc.rights | All rights reserved | en_US |
| dc.title | The setup and evaluation of a high performance liquid chromatography procedure for plasma homocysteine analysis and its application in determining typical values and age- and sex- related differences in a sample of apparently healthy local Chinese subjects | en_US |
| dcterms.abstract | Background: Plasma homocysteine (Hcy) is an independent emerging cardiovascular disease (CVD) risk biomarker. Its measurement together with well-established traditional CVD risk biomarkers such as blood cholesterol is clinically useful in better assessing CVD risk. Therefore, a sensitive and specific method is needed for Hcy measurement. Aims: To 1. set up and evaluate of a high performance liquid chromatography (HPLC) procedure for plasma homocysteine (Hcy) analysis. 2. determine typical values and investigate of age- and sex- related differences of plasma Hcy in a sample of apparently healthy local Chinese subjects. Results: An HPLC with fluorescence detection (HPLC-FL) for plasma total homocysteine (tHcy) analysis was set up. After pre-treatment procedures, injection of a 10 μL derivatized sample onto the HPLC-FL system was performed. The analysis time per sample is 10 minutes. The method proved to be linear from 0 to 100 μmol/L (r² = 0.9999). The within-run and between-run coefficient of variations were 0.5%-0.8% and 2.8%-4.6% respectively at each of 3 different Hcy levels (5, 10 and 50 μmol/L, n = 6 for each). The detection reproducibility of plasma tHcy at 4℃ over 15 hours was 0.7%-2.0% (n = 11) across 3 different Hcy levels (5, 10 and 50 μmol/L). The mean recovery was 94.5%-102.8% across 3 different Hcy levels (5, 10 and 50 μmol/L, n = 6 for each). The limit of detection and the limit of quantitation in plasma matrix were 0.36 μmol/L and 0.73 μmol/L respectively. The range of plasma tHcy in 197 apparently healthy adults was 3.6 to 50.7 μmol/L. The median, mean (SD) and range of plasma tHcy for men (n = 86) were found to be 9.7, 10.9 (5.8) and 5.3-50.7 μmol/L respectively. The median, mean (SD) and range of plasma tHcy for women (n = 111) were found to be 7.2, 7.2 (1.6) and 3.6-12.0 μmol/L respectively. The plasma tHcy in men with a median of 9.7 μmol/L was significantly higher (p < 0.0001) than that of women with a median plasma tHcy of 7.2 μmol/L. The median, mean (SD) and range of plasma tHcy for the age group 20-35 years (n = 57) were found to be 8.6, 10.3 (6.9) and 3.6-50.7 μmol/L respectively. The median, mean (SD) and range of plasma tHcy for the age group 36-50 years (n = 63) were found to be 7.6, 8.2 (2.5) and 4.5-20.7 μmol/L respectively. The median, mean (SD) and range of plasma tHcy for the age group 51-65 years (n = 77) were found to be 7.7, 8.2 (2.6) and 4.5-17.5 μmol/L respectively. The medians of plasma tHcy of 8.6, 7.6 and 7.7 μmol/L for the different age groups were not significantly different (p = 0.0777) from each other. The range covering 95% of results was 5.3-17.3 μmol/L for men and 3.6-10.2 μmol/L for women. | en_US |
| dcterms.abstract | Conclusion: A rapid, reliable, sensitive and specific HPLC-FL for plasma tHcy analysis was developed. It gives a documented evaluated methodology for plasma tHcy measurement in our Health Technology and Informatics Biomedical Science reference laboratory. In a sample of 197 apparently healthy adults in our local population, men had higher plasma tHcy levels than women. Interestingly, taking a cut-off of "desirable" plasma tHcy of 10 μmol/L, 39 (45%) men and 7 (6%) women had high levels, indicating elevated CVD risk. Eight (9%) men but no women had clearly elevated plasma tHcy levels (> 15 μmol/L), indicating significantly elevated CVD risk. Results indicate that health promotion for CVD risk reduction in the general population is needed. | en_US |
| dcterms.extent | xiii, 125 leaves : ill. ; 31 cm. | en_US |
| dcterms.isPartOf | PolyU Electronic Theses | en_US |
| dcterms.issued | 2010 | en_US |
| dcterms.educationalLevel | All Master | en_US |
| dcterms.educationalLevel | M.Sc. | en_US |
| dcterms.LCSH | Hong Kong Polytechnic University -- Dissertations | en_US |
| dcterms.LCSH | Homocysteine -- Pathophysiology | en_US |
| dcterms.LCSH | Liquid chromatography | en_US |
| dcterms.LCSH | Blood plasma | en_US |
| dcterms.LCSH | Cardiovascular system -- Diseases -- Risk factors. | en_US |
| dcterms.accessRights | restricted access | en_US |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| b23559603.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 891.1 kB | Adobe PDF | View/Open |
Copyright Undertaking
As a bona fide Library user, I declare that:
- I will abide by the rules and legal ordinances governing copyright regarding the use of the Database.
- I will use the Database for the purpose of my research or private study only and not for circulation or further reproduction or any other purpose.
- I agree to indemnify and hold the University harmless from and against any loss, damage, cost, liability or expenses arising from copyright infringement or unauthorized usage.
By downloading any item(s) listed above, you acknowledge that you have read and understood the copyright undertaking as stated above, and agree to be bound by all of its terms.
Please use this identifier to cite or link to this item:
https://theses.lib.polyu.edu.hk/handle/200/5842