| Author: | Chan, Chun Yu Ron |
| Title: | Polyethylene-glycol hydrogel scaffold conjugated with cell adhesion peptide for cell culture |
| Degree: | M.Sc. |
| Year: | 2012 |
| Subject: | Polyethylene glycol. Cell adhesion. Hong Kong Polytechnic University -- Dissertations |
| Department: | Department of Health Technology and Informatics |
| Pages: | xiv, 50 leaves : ill. (some col.) ; 30 cm. |
| Language: | English |
| Abstract: | Results of nerve damage various; including loss of sensation, motor movement impairment, which depends on the site of incision. Traditionally, nerve severance was treated by autograph, requiring the surgeon to extract another segment of nerve from the patient. This will create another opening wound on the patient and the healing of nerve may not be satisfying. Tissue engineering is a very hot research topic providing new cues in treating nerve severance. Nerve guidance conduit, which is basically a cell culture scaffold, could help to guide and stimulate nerve regeneration. Poly (ethylene-glycol) hydrogel, which is highly hydrophilic, biocompatible, non-toxic and wildly applied in medical treatment, was considered as a potential material for fabricating nerve guidance conduit. It is often described as a "blank slate", while properties can be "written" on it. The tunable characteristic made it very suitable for being a nerve guidance conduit. In this study, cell adhesion peptide, a component in extracellular matrix, was immobilized in poly (ethylene-glycol). The cell adhesion peptide provided binding site for integrin receptors on cell surface, allowing cells to bind on a hydrophilic hydrogel. Experimental result demonstrated that when the cell adhesion peptide concentration in hydrogel reached 5.3μmol/cm³, neurite extension activity was found on cells seeded on it. This exploratory study verified the capability of cell adhesion peptide modified four-arm poly (ethylene-glycol) hydrogel of being a cell culturing scaffold which can support neurite extension. In future, it could become a wildly used tissue engineering scaffold thanks for its biocompatibility. |
| Rights: | All rights reserved |
| Access: | restricted access |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| b25202133.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 1.69 MB | Adobe PDF | View/Open |
Copyright Undertaking
As a bona fide Library user, I declare that:
- I will abide by the rules and legal ordinances governing copyright regarding the use of the Database.
- I will use the Database for the purpose of my research or private study only and not for circulation or further reproduction or any other purpose.
- I agree to indemnify and hold the University harmless from and against any loss, damage, cost, liability or expenses arising from copyright infringement or unauthorized usage.
By downloading any item(s) listed above, you acknowledge that you have read and understood the copyright undertaking as stated above, and agree to be bound by all of its terms.
Please use this identifier to cite or link to this item:
https://theses.lib.polyu.edu.hk/handle/200/6557