| Author: | Meng, Nuo Ya |
| Title: | Graphene oxide based on fluorescence resonance energy transfer (FREF) biosensor for H5N1 DNA detection |
| Degree: | M.Sc. |
| Year: | 2014 |
| Subject: | Avian influenza Avian influenza -- Diagnosis. Hong Kong Polytechnic University -- Dissertations |
| Department: | Interdisciplinary Division of Biomedical Engineering |
| Pages: | 58 leaves : illustrations (chiefly color) ; 30 cm |
| Language: | English |
| Abstract: | Human inflection with H5N1 avian flu has been found since 1997 and over 500 people infected by this virus. It is urgent to find a rapid and novel method to detect H5N1 virus with high efficiency and sensitivity. Based on absorption of graphene oxide (GO) with single-stranded DNA (ssDNA), a FRET biosensor based on FREF for H5N1 virus DNA has been developed. In our study, the H5N1 virus DNA was binding with fluorescent dye 5′6-FAM (5′6-carboxyfluorescein) as the source of fluorescence. When adding graphene oxide aqueous solution, H5N1 virus DNA was adsorbed to graphene oxide surface and the fluorescence of dye was quenched by graphene oxide. Subsequently, a short complementary DNA probe was utilized to specifically hybrid with H5N1 virus DNA, which leads the fluorescent dye FAM with double stranded DNA (dsDNA) released from graphene oxide surface, which results in recovery of the fluorescence. Experiment with non-complimentary DNA was explored for testify the specificity. The limit of detection of this GO based FRET biosensor is around 0.015uM. These results suggest the novel GO based FRET biosensor supplies a rapid approach for early H5N1 virus DNA diagnosis with high sensitivity and specificity. |
| Rights: | All rights reserved |
| Access: | restricted access |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| b27577399.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 1.69 MB | Adobe PDF | View/Open |
Copyright Undertaking
As a bona fide Library user, I declare that:
- I will abide by the rules and legal ordinances governing copyright regarding the use of the Database.
- I will use the Database for the purpose of my research or private study only and not for circulation or further reproduction or any other purpose.
- I agree to indemnify and hold the University harmless from and against any loss, damage, cost, liability or expenses arising from copyright infringement or unauthorized usage.
By downloading any item(s) listed above, you acknowledge that you have read and understood the copyright undertaking as stated above, and agree to be bound by all of its terms.
Please use this identifier to cite or link to this item:
https://theses.lib.polyu.edu.hk/handle/200/7554