| Author: | Tse, Wing Lam Wynne |
| Title: | Comparison of real-time quantitative PCR and droplet digital PCR for HIV viral load quantification |
| Advisors: | Leung, Polly (HTI) |
| Degree: | M.Sc. |
| Year: | 2016 |
| Subject: | AIDS (Disease) -- Laboratory manuals. HIV (Viruses) -- Laboratory manuals. Hong Kong Polytechnic University -- Dissertations |
| Department: | Department of Health Technology and Informatics |
| Pages: | vii, 40 pages : color illustrations |
| Language: | English |
| Abstract: | Assessment of HIV viral load is one of the best predictors of clinical disease progression and it is the main parameter used to evaluate treatment success in HIV-positive patients. Maintenance of an undetectable viral load is fundamental so as to know we are moving in the right direction towards long-term HIV suppression and even eradication in the battle with HIV. Otherwise, inappropriate clinical management as well as the selection and accumulation of residual drug-resistant viruses and subsequent transmission within the population could result in inevitable consequences. In the present study, the performance of real-time quantitative PCR (qPCR) and droplet digital PCR (ddPCR) for HIV viral load quantification was evaluated. Plasmid DNA extracted from pNL4-3 and RNA from the Qnostics HIV-1 Evaluation Panel was used for evaluation. The result of the novel platform, ddPCR, found no better than qPCR, the most frequently used method currently. The proposed improvement in accuracy, sensitivity and precision for low input target concentration over qPCR would await further approval. |
| Rights: | All rights reserved |
| Access: | restricted access |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| b2925467x.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 1.63 MB | Adobe PDF | View/Open |
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