Full metadata record
DC FieldValueLanguage
dc.contributorMulti-disciplinary Studiesen_US
dc.contributorDepartment of Applied Biology and Chemical Technologyen_US
dc.creatorWong, Ming-yan-
dc.identifier.urihttps://theses.lib.polyu.edu.hk/handle/200/937-
dc.languageEnglishen_US
dc.publisherHong Kong Polytechnic University-
dc.rightsAll rights reserveden_US
dc.titlePolyhydroxyalkanoates production by recombinant E. coli BL1-Blue Ampr, E. coli HMS174 Ampr, A. latus DSM1123 and A. latus DSM1124 using medium chain length fatty acid as sole carbon sourceen_US
dcterms.abstractTo investigate the ability of recombinant E. coli BL1-Blue Ampr , E. coli HMS 174 Ampr, Alcaligenes latus DSM1123 and Alcaligenes latus DSM1124 to utilize medium chain length fatty acid, capric and oleic acid as sole carbon source for PHA production, the strains were cultivated at 37C orbital shaker in l00mL medium E (Cromwick, Foglia, Lenz, 1996) containing 0.2% w/v capric, 0.2%, 0.5% and 1.0% w/v oleic acid. All the four strains of the bacteria were found sustainable in the medium mentioned above with different grow and PHA production characteristics. Fed batch fermentation was more effective for both cell grow and PHA production. It was found that Alcaligenes latus DSM1124 produced the highest amount of PHB in 1.0%w/v oleic acid medium. The amount of PHB produced was 4.43mg from l00mL medium. Reducing the concentration of the medium would reducing the amount of PHB produced. 0.2% w/v oleic acid medium was not sufficient to support cell grow for the four strains of bacteria due to nutrient depletion. The finding was different from capri acid, PHB and PHV was produced by all the four strains of bacteria in capric acid medium with the concentration as low as 0.2% w/v. The amount of PHB produced from recombinant E.coil BLi-Blue Ampr was 0.71mg, 0.36% product yield, 0.50mg, 0.25% product yield from recombinant E. coli HMS174 Ampr, 0.53mg, 0.27% product yield from A. latus DSM1123 and 1.29mg, 0.65% product yield from A. latus DSM1124 in l00mL 0.2% w/v medium each. The result showed that capric acid was more effective for PHA production.en_US
dcterms.extentxiii, 72 leaves : ill. ; 30 cmen_US
dcterms.isPartOfPolyU Electronic Thesesen_US
dcterms.issued2002en_US
dcterms.educationalLevelAll Masteren_US
dcterms.educationalLevelM.Sc.en_US
dcterms.LCSHHong Kong Polytechnic University -- Dissertationsen_US
dcterms.LCSHPoly-beta-hydroxyalkanoatesen_US
dcterms.accessRightsrestricted accessen_US

Files in This Item:
File Description SizeFormat 
b16154484.pdfFor All Users (off-campus access for PolyU Staff & Students only)4.39 MBAdobe PDFView/Open


Copyright Undertaking

As a bona fide Library user, I declare that:

  1. I will abide by the rules and legal ordinances governing copyright regarding the use of the Database.
  2. I will use the Database for the purpose of my research or private study only and not for circulation or further reproduction or any other purpose.
  3. I agree to indemnify and hold the University harmless from and against any loss, damage, cost, liability or expenses arising from copyright infringement or unauthorized usage.

By downloading any item(s) listed above, you acknowledge that you have read and understood the copyright undertaking as stated above, and agree to be bound by all of its terms.

Show simple item record

Please use this identifier to cite or link to this item: https://theses.lib.polyu.edu.hk/handle/200/937