| Author: | Yum, Kee-him |
| Title: | Diffusion controllable system with nanoporous alumina membrane |
| Degree: | M.Sc. |
| Year: | 2010 |
| Subject: | Hong Kong Polytechnic University -- Dissertations Aluminum oxide Porous materials Membrane filters Nanomedicine. |
| Department: | Department of Health Technology and Informatics |
| Pages: | ix, 63 leaves : ill. (some col.) ; 30 cm. |
| Language: | English |
| Abstract: | This thesis demonstrates the use of nanoporous alumina membranes for diffusion of some protein based biomolecules, including bovine serum albumin (BSA), insulin and one kind of anti-cancer drug, cisplatin (CDDP). With the construction of a polydimethylsiloxane (PDMS) chamber, the nanoporous membrane having 20 nm and 100 nm diameter is used as a diffusion controlling system. The diffusion properties are being investigated and compared with the two membrane pore size. The three biomolecules with different concentration is placed in to the chamber with a "filter" of the membrane, and allowed sufficient time for diffusion from one side to another side. The diffusion properties can be monitored by UV-visible spectroscopy since protein has maxima of absorption peak in 280 nm wavelength and CDDP has a range between 280 nm to 300 nm. The results would show the relationship of diffusion rate versus to nanopore size of the membrane and the concentration of certain biomolecules. To further investigate the diffusive effects of the biomolecules, some impedance testing have been done with the cancer cell line KYSE30 (from human esophageal squamous epithelial cancer cell). In the whole experiments, it herein serves as an exploratory test for the application of nanoporous alumina in novel drug delivery system. |
| Rights: | All rights reserved |
| Access: | restricted access |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| b23526750.pdf | For All Users (off-campus access for PolyU Staff & Students only) | 2.18 MB | Adobe PDF | View/Open |
Copyright Undertaking
As a bona fide Library user, I declare that:
- I will abide by the rules and legal ordinances governing copyright regarding the use of the Database.
- I will use the Database for the purpose of my research or private study only and not for circulation or further reproduction or any other purpose.
- I agree to indemnify and hold the University harmless from and against any loss, damage, cost, liability or expenses arising from copyright infringement or unauthorized usage.
By downloading any item(s) listed above, you acknowledge that you have read and understood the copyright undertaking as stated above, and agree to be bound by all of its terms.
Please use this identifier to cite or link to this item:
https://theses.lib.polyu.edu.hk/handle/200/5624