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dc.contributorInterdisciplinary Division of Biomedical Engineeringen_US
dc.creatorYang, Suhan-
dc.publisherHong Kong Polytechnic University-
dc.rightsAll rights reserveden_US
dc.titleDNA absorption and desorption with functionalized nanoporous anodic aluminum oxide membraneen_US
dcterms.abstractDNA extraction and purification from vivo sample is demanded in various research fields for genetic analysis process. Characterized by its chemical stability, potentials in diversified surface modification options and simplified protocols, solid-phase based method for DNA molecules isolation has widely discussed. In this project, a novel biomembrane: nanoporous anodic aluminum oxide (AAO) membrane was employed as DNA extraction substrate after APTES modification to investigate its optimal interaction conditions and to exam the different Lambda DNA binding capacities among three pore sizes. The best absorption and desorption conditions were pH 4.5 and pH 10.5, under these reaction conditions, Lambda DNA capturing ability increased with the amino group intensity.en_US
dcterms.extentix, 49 leaves : ill. (some col.) ; 30 cm.en_US
dcterms.educationalLevelAll Masteren_US
dcterms.LCSHMobile genetic elements.en_US
dcterms.LCSHDNA -- drug effects.en_US
dcterms.LCSHHong Kong Polytechnic University -- Dissertationsen_US
dcterms.accessRightsrestricted accessen_US

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